Excitatory amino acid transporter 2

Excitatory amino acid transporter 2 (EAAT2) also known as solute carrier family 1 member 2 (SLC1A2) and glutamate transporter 1 (GLT-1) is a protein that in humans is encoded by the SLC1A2 gene.[5][6] Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.[6]

SLC1A2
Identifiers
AliasesSLC1A2, EAAT2, GLT-1, HBGT, solute carrier family 1 member 2, EIEE41, DEE41
External IDsOMIM: 600300 MGI: 101931 HomoloGene: 3075 GeneCards: SLC1A2
Orthologs
SpeciesHumanMouse
Entrez

6506

20511

Ensembl

ENSG00000110436

ENSMUSG00000005089

UniProt

P43004

P43006

RefSeq (mRNA)

NM_001195728
NM_001252652
NM_004171

NM_001077514
NM_001077515
NM_011393
NM_001361018

RefSeq (protein)

NP_001182657
NP_001239581
NP_004162

NP_001070982
NP_001070983
NP_035523
NP_001347947

Location (UCSC)Chr 11: 35.25 – 35.42 MbChr 2: 102.49 – 102.62 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Function

SLC1A2 / EAAT2 is a member of a family of the solute carrier family of proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors.[6] EAAT2 is responsible for over 90% of glutamate reuptake within the brain.[7][8]

EAAT2 reuptake diagram
This diagram shows the tissue distribution of glutamate transporter 1 (EAAT2) in the brain.[7]

Clinical significance

Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis (ALS).[6] The drug riluzole approved for the treatment of ALS upregulates EAAT2.[9]

Ceftriaxone, an antibiotic, has been shown to induce/enhance the expression of EAAT2, resulting in reduced glutamate activity.[10] Ceftriaxone has been shown to reduce the development and expression of tolerance to opiates and other drugs of abuse. EAAT2 may possess an important role in drug addiction and tolerance to addictive drugs.[11]

Upregulation of EAAT2 (GLT-1) causes impairment of prepulse inhibition, a sensory gating deficit present in schizophrenics and schizophrenia animal models.[12][13] Some antipsychotics have been shown to reduce the expression of EAAT2.[14][15]

Interactions

SLC1A2 has been shown to interact with JUB.[16]

As a drug target

EAAT2/GLT-1, being the most abundant subtype of glutamate transporter in the CNS, plays a key role in regulation of glutamate neurotransmission. Dysfunction of EAAT2 has been correlated with various pathologies such as traumatic brain injury, stroke, Amyotrophic lateral sclerosis (ALS), Alzheimer's disease, among others. Therefore, activators of the function or enhancers of the expression of EAAT2/GLT-1 could serve as a potential therapy for these conditions. Translational activators of EAAT2/GLT-1, such as ceftriaxone and LDN/OSU-0212320, have been described to have significant protective effects in animal models of ALS and epilepsy. In addition, pharmacological activators of the activity of EAAT2/GLT-1 have been explored for decades and are currently emerging as promising tools for neuroprotection, having potential advantages over expression activators.[17]

DL-TBOA, WAY-213,613, and dihydrokainic acid are known inhibitors of the protein, and function as excitotoxins. They can be considered a novel class of nerve agent toxins, inducing toxic levels of glutamate through transport inhibition in a manner analogous to the effect of sarin on cholinesterase. Antidotes for such a poisoning have never been formally tested for efficacy and are not readily available for medical use.[18]

Addiction to certain drugs (e.g., cocaine, heroin, alcohol, and nicotine) is correlated with a persistent reduction in the expression of EAAT2 in the nucleus accumbens (NAcc);[19] the reduced expression of EAAT2 in this region is implicated in addictive drug-seeking behavior.[19] In particular, the long-term dysregulation of glutamate neurotransmission in the NAcc of addicts is associated with an increase in vulnerability to relapse after re-exposure to the addictive drug or its associated drug cues.[19] Drugs which help to normalize the expression of EAAT2 in this region, such as N-acetylcysteine, have been proposed as an adjunct therapy for the treatment of addiction to cocaine, nicotine, alcohol, and other drugs.[19]

See also

References
  1. GRCh38: Ensembl release 89: ENSG00000110436 - Ensembl, May 2017
  2. GRCm38: Ensembl release 89: ENSMUSG00000005089 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. Pines G, Danbolt NC, Bjørås M, Zhang Y, Bendahan A, Eide L, Koepsell H, Storm-Mathisen J, Seeberg E, Kanner BI (Dec 1992). "Cloning and expression of a rat brain L-glutamate transporter". Nature. 360 (6403): 464–7. Bibcode:1992Natur.360..464P. doi:10.1038/360464a0. PMID 1448170. S2CID 4243369.
  6. "Entrez Gene: SLC1A2 solute carrier family 1 (glial high affinity glutamate transporter), member 2".
  7. Rao P, Yallapu MM, Sari Y, Fisher PB, Kumar S (July 2015). "Designing Novel Nanoformulations Targeting Glutamate Transporter Excitatory Amino Acid Transporter 2: Implications in Treating Drug Addiction". J. Pers. Nanomed. 1 (1): 3–9. PMC 4666545. PMID 26635971. The glutamate transporter 1 (GLT1)/ excitatory amino acid transporter 2 (EAAT2) is responsible for the reuptake of more than 90% glutamate in the CNS [12–14].
  8. Holmseth S; Scott HA; Real K; Lehre KP; Leergaard TB; Bjaalie JG; Danbolt NC (2009). "The concentrations and distributions of three C-terminal variants of the GLT1 (EAAT2; slc1a2) glutamate transporter protein in rat brain tissue suggest differential regulation". Neuroscience. 162 (4): 1055–71. doi:10.1016/j.neuroscience.2009.03.048. PMID 19328838. S2CID 41615013. Since then, a family of five high-affinity glutamate transporters has been characterized that is responsible for the precise regulation of glutamate levels at both synaptic and extrasynaptic sites, although the glutamate transporter 1 (GLT1) is responsible for more than 90% of glutamate uptake in the brain.3 The importance of GLT1 is further highlighted by the large number of neuropsychiatric disorders associated with glutamate-induced neurotoxicity.

    Clarification of nomenclature
    The major glial glutamate transporter is referred to as GLT1 in the rodent literature and excitatory amino acid transporter 2 (EAAT2) in the human literature.
  9. Carbone M, Duty S, Rattray M (2012). "Riluzole elevates GLT-1 activity and levels in striatal astrocytes". Neurochem. Int. 60 (1): 31–8. doi:10.1016/j.neuint.2011.10.017. PMC 3430367. PMID 22080156.
  10. Lee SG, Su ZZ, Emdad L, Gupta P, Sarkar D, Borjabad A, Volsky DJ, Fisher PB (May 2008). "Mechanism of ceftriaxone induction of excitatory amino acid transporter-2 expression and glutamate uptake in primary human astrocytes". J. Biol. Chem. 283 (19): 13116–23. doi:10.1074/jbc.M707697200. PMC 2442320. PMID 18326497.
  11. Reissner KJ, Kalivas PW (2010). "Using glutamate homeostasis as a target for treating addictive disorders". Behav Pharmacol. 21 (5–6): 514–22. doi:10.1097/FBP.0b013e32833d41b2. PMC 2932669. PMID 20634691.
  12. Bellesi M, Melone M, Gubbini A, Battistacci S, Conti F (2009). "GLT-1 upregulation impairs prepulse inhibition of the startle reflex in adult rats". Glia. 57 (7): 703–13. doi:10.1002/glia.20798. PMID 18985735. S2CID 3222131.
  13. Bellesi M, Conti F (2010). "The mGluR2/3 agonist LY379268 blocks the effects of GLT-1 upregulation on prepulse inhibition of the startle reflex in adult rats". Neuropsychopharmacology. 35 (6): 1253–60. doi:10.1038/npp.2009.225. PMC 3055342. PMID 20072121.
  14. Schmitt A, Zink M, Petroianu G, May B, Braus DF, Henn FA (2003). "Decreased gene expression of glial and neuronal glutamate transporters after chronic antipsychotic treatment in rat brain". Neurosci. Lett. 347 (2): 81–4. doi:10.1016/S0304-3940(03)00653-0. PMID 12873733. S2CID 43706291.
  15. Vallejo-Illarramendi A, Torres-Ramos M, Melone M, Conti F, Matute C (2005). "Clozapine reduces GLT-1 expression and glutamate uptake in astrocyte cultures". Glia. 50 (3): 276–9. doi:10.1002/glia.20172. PMID 15739191. S2CID 18972974.
  16. Marie H, Billups D, Bedford FK, Dumoulin A, Goyal RK, Longmore GD, Moss SJ, Attwell D (February 2002). "The amino terminus of the glial glutamate transporter GLT-1 interacts with the LIM protein Ajuba". Mol. Cell. Neurosci. 19 (2): 152–64. doi:10.1006/mcne.2001.1066. PMID 11860269. S2CID 45768895.
  17. Fontana AC (June 20, 2015). "Current approaches to enhance glutamate transporter function and expression". Journal of Neurochemistry. 134 (6): 982–1007. doi:10.1111/jnc.13200. PMID 26096891.
  18. KEIKO SHIMAMOTO, BRUNO LEBRUN, YOSHIMI YASUDA-KAMATANI, MASAHIRO SAKAITANI, YASUSHI SHIGERI, NOBORU YUMOTO, and TERUMI NAKAJIMA (February 1998). "DL-threo-b-Benzyloxyaspartate, A Potent Blocker of Excitatory Amino Acid Transporters" (PDF). Molecular Pharmacology. 53 (2): 195–201. doi:10.1124/mol.53.2.195. PMID 9463476.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  19. McClure EA, Gipson CD, Malcolm RJ, Kalivas PW, Gray KM (2014). "Potential role of N-acetylcysteine in the management of substance use disorders". CNS Drugs. 28 (2): 95–106. doi:10.1007/s40263-014-0142-x. PMC 4009342. PMID 24442756.

Further reading

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