STX8

Syntaxin-8 is a protein that in humans is encoded by the STX8 gene.[5][6][7] Syntaxin 8 directly interacts with HECTd3 and has similar subcellular localization.[8] The protein has been shown to form the SNARE complex with syntaxin 7, vti1b and endobrevin. These function as the machinery for the homotypic fusion of late endosomes.[9]

STX8
Identifiers
AliasesSTX8, CARB, syntaxin 8
External IDsOMIM: 604203 MGI: 1890156 HomoloGene: 37973 GeneCards: STX8
Orthologs
SpeciesHumanMouse
Entrez

9482

55943

Ensembl

ENSG00000170310

ENSMUSG00000020903

UniProt

Q9UNK0

O88983

RefSeq (mRNA)

NM_004853

NM_018768
NM_001356314
NM_001361350
NM_001361351
NM_001361352

RefSeq (protein)

NP_004844

NP_061238
NP_001343243
NP_001348279
NP_001348280
NP_001348281

Location (UCSC)Chr 17: 9.25 – 9.58 MbChr 11: 67.86 – 68.1 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Model organisms

Model organisms have been used in the study of STX8 function. A conditional knockout mouse line, called Stx8tm2a(EUCOMM)Wtsi[14][15] was generated as part of the International Knockout Mouse Consortium program—a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists—at the Wellcome Trust Sanger Institute.[16][17][18] Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[12][19] Twenty four tests were carried out on homozygous mutant adult mice, however no significant abnormalities were observed.[12]

Interactions

STX8 has been shown to interact with Vesicle-associated membrane protein 8,[20] VTI1B[21][22] and STX7.[20]

References
  1. GRCh38: Ensembl release 89: ENSG00000170310 - Ensembl, May 2017
  2. GRCm38: Ensembl release 89: ENSMUSG00000020903 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. Steegmaier M, Yang B, Yoo JS, Huang B, Shen M, Yu S, Luo Y, Scheller RH (Dec 1998). "Three novel proteins of the syntaxin/SNAP-25 family". The Journal of Biological Chemistry. 273 (51): 34171–9. doi:10.1074/jbc.273.51.34171. PMID 9852078.
  6. Thoreau V, Bergès T, Callebaut I, Guillier-Gencik Z, Gressin L, Bernheim A, Karst F, Mornon JP, Kitzis A, Chomel JC (Apr 1999). "Molecular cloning, expression analysis, and chromosomal localization of human syntaxin 8 (STX8)". Biochemical and Biophysical Research Communications. 257 (2): 577–83. doi:10.1006/bbrc.1999.0503. PMID 10198254.
  7. "Entrez Gene: STX8 syntaxin 8".
  8. Zhang L, Kang L, Bond W, Zhang N (Feb 2009). "Interaction between syntaxin 8 and HECTd3, a HECT domain ligase". Cellular and Molecular Neurobiology. 29 (1): 115–21. doi:10.1007/s10571-008-9303-0. PMID 18821010. S2CID 23216243.
  9. Kasai K, Suga K, Izumi T, Akagawa K (October 2008). "Syntaxin 8 has two functionally distinct di-leucine-based motifs". Cellular & Molecular Biology Letters. 13 (1): 144–54. doi:10.2478/s11658-007-0043-9. PMC 6275627. PMID 17965969.
  10. "Salmonella infection data for Stx8". Wellcome Trust Sanger Institute.
  11. "Citrobacter infection data for Stx8". Wellcome Trust Sanger Institute.
  12. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88 (S248). doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
  13. Mouse Resources Portal, Wellcome Trust Sanger Institute.
  14. "International Knockout Mouse Consortium".
  15. "Mouse Genome Informatics".
  16. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
  17. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
  18. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
  19. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biology. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.
  20. Antonin W, Holroyd C, Fasshauer D, Pabst S, Von Mollard GF, Jahn R (Dec 2000). "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". The EMBO Journal. 19 (23): 6453–64. doi:10.1093/emboj/19.23.6453. PMC 305878. PMID 11101518.
  21. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
  22. Subramaniam VN, Loh E, Horstmann H, Habermann A, Xu Y, Coe J, Griffiths G, Hong W (Mar 2000). "Preferential association of syntaxin 8 with the early endosome". Journal of Cell Science. 113 ( Pt 6) (6): 997–1008. doi:10.1242/jcs.113.6.997. PMID 10683148.

Further reading
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