Eosin

Eosin is the name of several fluorescent acidic compounds which bind to and form salts with basic, or eosinophilic, compounds like proteins containing amino acid residues such as arginine and lysine, and stains them dark red or pink as a result of the actions of bromine on eosin. In addition to staining proteins in the cytoplasm, it can be used to stain collagen and muscle fibers for examination under the microscope. Structures, that stain readily with eosin, are termed eosinophilic. In the field of histology, Eosin Y is the form of eosin used most often as a histologic stain.[1][2]

Eosin Y
Eosin B

Etymology

Eosin was named by its inventor Heinrich Caro after the nickname (Eos) of a childhood friend, Anna Peters.[3]

Variants
Eosin Y solution for staining microscopy slides.

There are actually two very closely related compounds commonly referred to as eosin. Most often used is in histology is Eosin Y[1][2] (also known as eosin Y ws, eosin yellowish, Acid Red 87, C.I. 45380, bromoeosine, bromofluoresceic acid, D&C Red No. 22); it has a very slightly yellowish cast. The other eosin compound is eosin B (eosin bluish, Acid Red 91, C.I. 45400, Saffrosine, Eosin Scarlet, or imperial red); it has a very faint bluish cast. The two dyes are interchangeable, and the use of one or the other is a matter of preference and tradition.

Eosin Y is a tetrabromo derivative of fluorescein.[4] Eosin B is a dibromo dinitro derivative of fluorescein.[5]

Uses

Use in histology
Eosinophilic staining, compared to other patterns when using hematoxylin and eosin (H&E).

Eosin is most often used as a counterstain to hematoxylin in H&E (haematoxylin and eosin) staining. H&E staining is one of the most commonly used techniques in histology. Tissue stained with haematoxylin and eosin shows cytoplasm stained pink-orange and nuclei stained darkly, either blue or purple. Eosin also stains red blood cells intensely red.

For staining, eosin Y is typically used in concentrations of 1 to 5 percent weight by volume, dissolved in water or ethanol.[6] For prevention of mold growth in aqueous solutions, thymol is sometimes added.[7] A small concentration (0.5 percent) of acetic acid usually gives a deeper red stain to the tissue.

It is listed as an IARC class 3 carcinogen.

Other uses
In his Field with Irises near Arles painting, Van Gogh incorporated red eosin dye into the color of the irises depicted at the bottom of the painting. Due to eosin's tendency to fade, the petals have now attained a blueish hue from their original purple coloration.[8]

Eosin is also used as a red dye in inks; however, the molecule, especially that of eosin Y, tends to degrade over time, leaving behind its bromine atoms, hence causing paint incorporating such a dye to obtain a darker brown tinge over time.[8] A notable user of eosin dye was the post-impressionist painter Van Gogh.

See also

References
  1. Lillie, Ralph Dougall (1977). H. J. Conn's Biological stains (9th ed.). Baltimore: Williams & Wilkins. pp. 692p.
  2. Bancroft, John; Stevens, Alan, eds. (1982). The Theory and Practice of Histological Techniques (2nd ed.). Longman Group Limited.
  3. Travis, Anthony S (1998). ""Ambitious and Glory Hunting . . . Impractical and Fantastic": Heinrich Caro at BASF". Technology and Culture. 39 (1): 105–115. doi:10.2307/3107005. JSTOR 3107005.
  4. Its CAS number is 17372-87-1 and its SMILES structure is O=C5C(Br)=C2O C1=C(Br)C([O-]) =C(Br)C=C1C(C4=C (C([O-])=O)C=C C=C4)=C2C=C3Br.
  5. Its CAS number is 548-28-3 and its SMILES structure is O=C5C(Br)=C2O C1=C(Br)C([O-]) =C([N+]([O-])=O) C=C1C(C4=C(C([O-]) =O)C=CC=C4)=C2 C=C3[N+]([O-])=O.
  6. "Haematoxylin Eosin (H&E) staining". protocolsonline.com. 11 April 2010. Retrieved 22 April 2018.
  7. Hitokoto H, Morozumi S, Wauke T, Sakai S, Kurata H (1980). "Inhibitory effects of spices on growth and toxin production of toxigenic fungi". Appl. Environ. Microbiol. 39 (4): 818–22. doi:10.1128/AEM.39.4.818-822.1980. PMC 291425. PMID 6769391.
  8. "Van Gogh's Fading Colors Inspire Scientific Inquiry".
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