How to Make Infusoria

Co-authored by Jessica Gibson

Last Updated: October 21, 2021 References Approved

Would you love to help your fish fry grow faster? Instead of buying nutritious microorganisms called infusoria, make your own with a little leftover produce. Combine a few pieces of leafy greens or vegetables in a jar with water from an established tank. Then leave it in the sunshine so the produce makes bacteria for the infusoria to feed on. Use an eye dropper to feed your fish fry a few drops of water that are rich with the infusoria and watch them grow!

Method 1

Method 1 of 2:

Culturing the Infusoria

1
Choose vegetables to make the infusoria starter. You can use canned or fresh vegetables as the base for the infusoria. If you like, use more than 1 type of vegetable, which will encourage a variety of microorganisms to grow. Consider using:^{[1]
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- Chopped lettuce or spinach leaves
- Shredded green cabbage
- Green beans
- Peas
2
Fill a 1 US quart (950 ml) jar 1/4 full with the vegetables. Chop the leafy greens into 1 to 2 inches (2.5 to 5.1 cm) pieces before you put them into the jar and shell green beans or peas. Put both the shells of the green beans and the peas into the jar.^{[2]
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- Chopping the vegetables will help it break down faster in the water.
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3
Fill the jar 1/3 full with boiling water and let it cool for 5 minutes. Bring a kettle of water to boil and carefully pour boiling water over the vegetables in the jar. Pour slowly until the jar is 1/3 full with boiling water. Then let the water cool until it's simply warm.^{[3]
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- The jar should be warm or at room temperature. If it's cold, it may shatter when you pour the boiling water into it.
4
Pour in aquarium water to fill the jar. Once the water in the jar is just warm to the touch, slowly pour in water from an established aquarium. Keep pouring until the jar is full of water.^{[4]
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- It's fine if a little debris from the aquarium gets into the jar.
- The water from the aquarium will have microorganisms that will help your infusoria grow.
5
Set the jar in a sunny place near a window. Move the jar to a windowsill where it will get sunshine throughout the day. While you don't need to seal the jar shut, you can place a light cloth, such as cheesecloth, over the top to prevent things from falling into the jar.
- Sunshine will encourage the vegetables to break down. This will create bacteria for the infusoria to eat.
6
Wait 2 to 3 days for the infusoria to develop. You'll notice the water become cloudy, which means that bacteria is starting to grow. As the infusoria grow, the water will become clearer because the infusoria is feeding on the bacteria. This is how you know the infusoria is ready to feed to your fish fry.^{[5]
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- Although cloudy water means the infusoria will soon be ready, don't use it until the water clears.
Tip: If you see scum form at the top of water, swirl the jar gently. This will ensure that oxygen reaches the material down in the jar.

Method 2

Method 2 of 2:

Using and Storing Infusoria

1
Use the dropper to feed the fry a few drops of infusoria twice a day. Insert a clean eye dropper or turkey baster into the middle of the jar with the infusoria. Remove some of the liquid and squirt a few drops into the fish tank that has your fry in it. Since the fry are growing quickly, feed them infusoria at least 2 times a day.^{[6]
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- Remember that you don't need to add very much liquid from the jar since infusoria are so small and there are a lot of them in a single drop of the liquid.
2
Feed the fry infusoria for about 1 week. Continue to feed the infusoria to your fish fry until they're big enough to start eating larger foods, such as brine shrimp nauplii. You should see the fry develop and grow quickly.^{[7]
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- If you put too much infusoria liquid into the tank with the fish fry, the water may turn cloudy.
Tip: If you'd like to have a batch of infusoria ready for when you discard your initial batch, start a new jar 3 to 4 days after starting the first.
3
Store the infusoria at room temperature. Keep the infusoria alive by avoiding extreme swings in temperature. Instead of storing the jar of infusoria in the refrigerator, leave it at room temperature. To slow down the development of bacteria and infusoria, remove the jar from the window and put it on a counter out of direct sunlight.^{[8]
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- To prevent things from falling into the jar, lay a piece of cheesecloth or a light towel over the top. Avoid screwing a lid tightly onto the jar because this will cause the food in the jar to spoil faster.
4
Discard the infusoria after 2 days. Once the infusoria has developed enough to feed to your fish fry, plan on using it within 2 days. After 2 days, the infusoria won't develop and bacteria will overwhelm the jar. This will make the water cloudy again. You'll also notice a rotting smell coming from the produce in the jar.^{[9]
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- Consider labeling the jar with the date you originally set it in the window, the date the infusoria was developed enough to use, and the date you need to discard it.

Community Q&A

Question

Can I use any plant instead of lettuce?

Community Answer

Yes, you can use almost any plant leaves that are not poisonous. For example, grass, tree leaves, and cucumber skin.
Question

What's the other alternative of algae water?

Community Answer

You can buy Copepod cultures at your LFS or online. Almost the same process, but you'll want to maintain your culture with aged aquarium water (after a water change) or rain water. Put the container you chose to use under direct light for 48-72 hours for bacteria and phytoplankton to grow for copepods to eat and you're set. Or, if you have a spacious, well-off tank, your fry can survive off of the ecosystem of the tank. I've left fry in the same tank as they were born, yes a couple were eaten, and they've grown into full adults in a 10 gallon tank.
Question

Can I use cabbage instead of lettuce?

Community Answer

Yes. I have used cabbage because I live in India and lettuce is not available here.