Sorting nexin
Sorting nexins are a large group of proteins that are localized in the cytoplasm and have the potential for membrane association either through their lipid-binding PX domain (a phospholipid-binding motif) or through protein–protein interactions with membrane-associated protein complexes[2][3] Some members of this family have been shown to facilitate protein sorting.
| PX domain | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 Structure of the PX domain from p40(phox) bound to phosphatidylinositol 3-phosphate.[1] | |||||||||
| Identifiers | |||||||||
| Symbol | PX | ||||||||
| Pfam | PF00787 | ||||||||
| InterPro | IPR001683 | ||||||||
| SMART | PX | ||||||||
| PROSITE | PDOC50195 | ||||||||
| SCOP2 | 1h6h / SCOPe / SUPFAM | ||||||||
| OPM superfamily | 57 | ||||||||
| OPM protein | 4on3 | ||||||||
| |||||||||
| Sorting nexin, N-terminal domain | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | Sorting_nexin | ||||||||
| Pfam | PF03700 | ||||||||
| InterPro | IPR005329 | ||||||||
| |||||||||
| Vps5 C terminal like (BAR domain) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | Vps5 | ||||||||
| Pfam | PF09325 | ||||||||
| InterPro | IPR015404 | ||||||||
| |||||||||
| Sorting nexin, C terminal domain | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | Nexin_C | ||||||||
| Pfam | PF08628 | ||||||||
| InterPro | IPR013937 | ||||||||
| |||||||||
| WASP-binding domain of Sorting nexin protein | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | BAR3-WASP | ||||||||
| Pfam | PF10456 | ||||||||
| |||||||||
Family members
In humans, sorting nexins are transcribed from the following genes:
| Genes | Domain Composition | ||||
|---|---|---|---|---|---|
| N-terminus | Mid-1 | Mid-2 | Mid-3 | C-terminus | |
| SNX1, SNX2 | Sorting_nexin_N | – | PX | – | BAR[4] (Vps5) |
| SNX4, SNX7, SNX8, SNX30 | – | – | PX | – | BAR[4] (Vsp5) |
| SNX5, SNX6, SNX32 | – | – | PX | – | BAR[4] |
| SNX9, SNX18, SNX33 | SH3 | – | PX | – | BAR[4] (BAR_3_WASP_bdg)[5] |
| SNX13, SNX14, SNX19, SNX25 | PXA[6] | RGS | PX | – | Nexin_C |
| SNX15 | – | – | PX | – | MIT[7] |
| SNX17, SNX31 | – | – | PX | RA[8] | FERM_M[9] |
| SNX23 | kinesin_motor | SMAD_FHA[10] | PX | – | – |
| SNX26 | – | – | PX | SH3 | RhoGAP |
| SNX27 | PDZ | – | PX | - | FERM_M[9] |
| SNX28 | – | – | PX | SH3 | SH3 |
| SNX3, SNX10, SNX11, SNX12, SNX16, SNX20, SNX21, SNX22, SNX24, SNX29 | – | – | PX | – | – |
Structure
Sorting nexins either consist solely of a PX domain (e.g. SNX3) or have a modular structure made up of the PX and additional domains.
A subgroup of sorting nexins (comprising, in humans, SNX1, SNX2, SNX4, SNX5, SNX6, SNX7, SNX8, SNX9, SNX18, SNX30, SNX32 and SNX33) possess a BAR domain at their C-terminus. (The BAR domain of SNXs 1, 2, 4, 7, 8 and 30 is classified by pfam as 'Vps5 C terminal like'.)
An example of a sorting nexin domain structure can be seen here for SNX1:
- NTD – N-terminal domain
- PX domain
- CTD – C-terminal BAR domain
References
- Bravo J, Karathanassis D, Pacold CM, et al. (October 2001). "The crystal structure of the PX domain from p40(phox) bound to phosphatidylinositol 3-phosphate". Mol. Cell. 8 (4): 829–39. doi:10.1016/S1097-2765(01)00372-0. PMID 11684018.
- Dixon JE, Worby CA (2002). "Sorting out the cellular functions of sorting nexins". Nat. Rev. Mol. Cell Biol. 3 (12): 919–931. doi:10.1038/nrm974. PMID 12461558. S2CID 36361630.
- Worby CA, Dixon JE (2002). "Sorting out the cellular functions of sorting nexins". Nat. Rev. Mol. Cell Biol. 3 (12): 919–31. doi:10.1038/nrm974. PMID 12461558. S2CID 36361630.
- Frost A, Unger VM, De Camilli P (April 2009). "The BAR domain superfamily: membrane-molding macromolecules". Cell. 137 (2): 191–196. doi:10.1016/j.cell.2009.04.010. PMC 4832598. PMID 19379681.
- BAR_3_WASP_bdg: WASP-binding domain of Sorting nexin protein, Pfam PF10456
- PXA: PXA domain, Pfam PF02194
- MIT: MIT (microtubule interacting and transport) domain, Pfam PF04212
- RA: Ras association (RalGDS/AF-6) domain, Pfam PF00788
- FERM_M: FERM central domain, Pfam PF00373
- SMAD_FHA: SMAD/FHA domain, InterPro: IPR008984
External links
- Sorting+Nexins at the U.S. National Library of Medicine Medical Subject Headings (MeSH)