Steroid 11β-hydroxylase

Steroid 11β-hydroxylase, also known as steroid 11β-monooxygenase, is a steroid hydroxylase found in the zona glomerulosa and zona fasciculata of the adrenal cortex. Named officially the cytochrome P450 11B1, mitochondrial, it is a protein that in humans is encoded by the CYP11B1 gene.[7][8] The enzyme is involved in the biosynthesis of adrenal corticosteroids[9] by catalyzing the addition of hydroxyl groups during oxidation reactions.

CYP11B1
Identifiers
AliasesCYP11B1, CPN1, CYP11B, FHI, P450C11, cytochrome P450 family 11 subfamily B member 1
External IDsOMIM: 610613 MGI: 88584 HomoloGene: 128035 GeneCards: CYP11B1
EC number1.14.15.4
Orthologs
SpeciesHumanMouse
Entrez

1584

13072

Ensembl

ENSG00000160882

ENSMUSG00000022589

UniProt

P15538

P15539

RefSeq (mRNA)

NM_000497
NM_001026213

NM_009991

RefSeq (protein)

NP_000488
NP_001021384

NP_034121

Location (UCSC)Chr 8: 142.87 – 142.88 MbChr 15: 74.72 – 74.73 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse
steroid 11β-monooxygenase
Identifiers
EC no.1.14.15.4
CAS no.9029-66-7
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins
CYP11B1
Identifiers
AliasesCYP11B1, CPN1, CYP11B, FHI, P450C11, cytochrome P450 family 11 subfamily B member 1
External IDsOMIM: 610613 MGI: 88584 HomoloGene: 128035 GeneCards: CYP11B1
EC number1.14.15.4
Orthologs
SpeciesHumanMouse
Entrez

1584

13072

Ensembl

ENSG00000160882

ENSMUSG00000022589

UniProt

P15538

P15539

RefSeq (mRNA)

NM_000497
NM_001026213

NM_009991

RefSeq (protein)

NP_000488
NP_001021384

NP_034121

Location (UCSC)Chr 8: 142.87 – 142.88 MbChr 15: 74.72 – 74.73 Mb
PubMed search[5][6]
Wikidata
View/Edit HumanView/Edit Mouse

Gene

The CYP11B1 gene encodes 11β-hydroxylase - a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The product of this CYP11B1 gene is the 11β-hydroxylase protein. This protein localizes to the mitochondrial inner membrane and is involved in the conversion of various steroids in the adrenal cortex. Transcript variants encoding different isoforms have been noted for this gene.[8]

The CYP11B1 gene is reversibly inhibited by etomidate[10][11] and metyrapone.

Function

11β-hydroxylase is a steroidogenic enzyme, i.e. the enzyme involved in the metabolism of steroids.

Humans have two isozymes with 11β-hydroxylase activity: CYP11B1 and CYP11B2.

CYP11B1 (11β-hydroxylase) is expressed at high levels and is regulated by ACTH, while CYP11B2 (aldosterone synthase) is usually expressed at low levels and is regulated by angiotensin II. In addition to the 11β-hydroxylase activity, both isozymes have 18-hydroxylase activity.[12] The CYP11B1 isozyme has strong 11β-hydroxylase activity, but the activity of 18-hydroxylase is only one-tenth of CYP11B2.[13] The weak 18-hydroxylase activity of CYP11B1 explains why an adrenal with suppressed CYP11B2 expression continues to synthesize 18-hydroxycorticosterone.[14]

Here are some of the steroids, grouped by catalytic activity of the CYP11B1 isozyme:

Cortisol and corticosterone metabolism

11β-hydroxylase has strong[15] catalytic activity during conversion of 11-deoxycortisol to cortisol and 11-deoxycorticosterone to corticosterone, by catalyzing the hydroxylation of carbon hydrogen bond at 11-beta position. Note the extra "–OH" added at the 11 position (near the center, on ring "C"):

Mechanism of action

As a mitochondrial P450 system, P450c11 is dependent on two electron transfer proteins, adrenodoxin reductase and adrenodoxin that transfer 2 electrons from NADPH to the P450 for each monooxygenase reaction catalyzed by the enzyme. In most respects this process of electron transfer appears similar to that of P450scc system that catalyzes cholesterol side chain cleavage.[27] Similar to P450scc the process of electrons transfer is leaky leading to superoxide production. The rate of electron leakage during metabolism depends on the functional groups of the steroid substrate.[28]

Regulation

The expression of the enzyme in adrenocortical cells is regulated by the trophic hormone corticotropin (ACTH).[29]

Clinical significance

A mutation in genes encoding 11β-hydroxylase is associated with congenital adrenal hyperplasia due to 11β-hydroxylase deficiency.

11β-hydroxylase is involved in the metabolism of 17α-hydroxyprogesterone to 21-deoxycortisol,[19] in cases of congenital adrenal hyperplasia due to 21-hydroxylase deficiency.[30]

See also

Additional images

Steroidogenesis, showing steroid 11-beta-hydroxylase vertically at right.

References

  1. GRCh38: Ensembl release 89: ENSG00000160882 - Ensembl, May 2017
  2. GRCm38: Ensembl release 89: ENSMUSG00000022589 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  6. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  7. Lifton RP, Dluhy RG, Powers M, Rich GM, Gutkin M, Fallo F, et al. (September 1992). "Hereditary hypertension caused by chimaeric gene duplications and ectopic expression of aldosterone synthase". Nature Genetics. 2 (1): 66–74. doi:10.1038/ng0992-66. hdl:11577/133580. PMID 1303253. S2CID 975796.
  8. "Entrez Gene: CYP11B1 cytochrome P450, family 11, subfamily B, polypeptide 1". National Center for Biotechnology Information. Retrieved 30 November 2020. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and is involved in the conversion of progesterone to cortisol in the adrenal cortex. Mutations in this gene cause congenital adrenal hyperplasia due to 11-beta-hydroxylase deficiency. Transcript variants encoding different isoforms have been noted for this gene.Public Domain This article incorporates text from this source, which is in the public domain.
  9. Zöllner A, Kagawa N, Waterman MR, Nonaka Y, Takio K, Shiro Y, et al. (February 2008). "Purification and functional characterization of human 11beta hydroxylase expressed in Escherichia coli". The FEBS Journal. 275 (4): 799–810. doi:10.1111/j.1742-4658.2008.06253.x. PMID 18215163. S2CID 45997341.
  10. Dörr HG, Kuhnle U, Holthausen H, Bidlingmaier F, Knorr D (November 1984). "Etomidate: a selective adrenocortical 11 beta-hydroxylase inhibitor". Klinische Wochenschrift. 62 (21): 1011–3. doi:10.1007/bf01711722. PMID 6096625. S2CID 20077711.
  11. Lake CL (7 December 2004). Pediatric Cardiac Anesthesia. Lippincott Williams & Wilkins. p. 68. ISBN 978-0-7817-5175-9. Retrieved 30 April 2012.
  12. White PC, Curnow KM, Pascoe L (August 1994). "Disorders of steroid 11 beta-hydroxylase isozymes". Endocrine Reviews. 15 (4): 421–38. doi:10.1210/edrv-15-4-421. PMID 7988480.
  13. White PC, Curnow KM, Pascoe L (1993). "Steroid 11β-Hydroxylase Isozymes (CYP11B1 and CYP11B2)". Cytochrome P450. Handbook of Experimental Pharmacology. Vol. 105. pp. 641–650. doi:10.1007/978-3-642-77763-9_41. ISBN 978-3-642-77765-3. S2CID 81304246.
  14. Gupta V (October 2011). "Mineralocorticoid hypertension". Indian Journal of Endocrinology and Metabolism. 15 Suppl 4 (8): S298–312. doi:10.4103/2230-8210.86972. PMC 3230101. PMID 22145132.
  15. Strushkevich N, Gilep AA, Shen L, Arrowsmith CH, Edwards AM, Usanov SA, Park HW (February 2013). "Structural insights into aldosterone synthase substrate specificity and targeted inhibition". Molecular Endocrinology. 27 (2): 315–24. doi:10.1210/me.2012-1287. PMC 5417327. PMID 23322723.
  16. van Rooyen D, Gent R, Barnard L, Swart AC (April 2018). "The in vitro metabolism of 11β-hydroxyprogesterone and 11-ketoprogesterone to 11-ketodihydrotestosterone in the backdoor pathway". The Journal of Steroid Biochemistry and Molecular Biology. 178: 203–212. doi:10.1016/j.jsbmb.2017.12.014. PMID 29277707. S2CID 3700135.
  17. Mello, Penachioni, Amaral, Castro (October 2004). "11beta-hydroxylase deficiency". Arquivos Brasileiros de Endocrinologia e Metabologia (in Portuguese). 48 (5): 713–23. doi:10.1590/s0004-27302004000500018. PMID 15761543.
  18. Bulsari K, Falhammar H (January 2017). "Clinical perspectives in congenital adrenal hyperplasia due to 11β-hydroxylase deficiency". Endocrine. 55 (1): 19–36. doi:10.1007/s12020-016-1189-x. PMID 27928728. S2CID 11153844.
  19. Turcu AF, Rege J, Chomic R, Liu J, Nishimoto HK, Else T, et al. (June 2015). "Profiles of 21-Carbon Steroids in 21-hydroxylase Deficiency". The Journal of Clinical Endocrinology and Metabolism. 100 (6): 2283–90. doi:10.1210/jc.2015-1023. PMC 4454804. PMID 25850025.
  20. Masiutin, Maxim; Yadav, Maneesh (2023). "Alternative androgen pathways". WikiJournal of Medicine. 10: X. doi:10.15347/WJM/2023.003. S2CID 257943362.
  21. Bloem LM, Storbeck KH, Schloms L, Swart AC (October 2013). "11β-hydroxyandrostenedione returns to the steroid arena: biosynthesis, metabolism and function". Molecules. MDPI AG. 18 (11): 13228–44. doi:10.3390/molecules181113228. PMC 6270415. PMID 24165582.
  22. Storbeck KH, Mostaghel EA (2019). "Canonical and Noncanonical Androgen Metabolism and Activity". Prostate Cancer. Advances in Experimental Medicine and Biology. Vol. 1210. pp. 239–277. doi:10.1007/978-3-030-32656-2_11. ISBN 978-3-030-32655-5. PMID 31900912. S2CID 209748543. CYP11B1 and 2 have also been shown to 11β-hydroxylate T, yielding 11β-hydroxytestosterone (11OHT), though the levels produced by the adrenal are low due to the limited availability of adrenal derived T
  23. Stárka L, Dušková M, Vítků J (September 2020). "11-Keto-testosterone and other androgens of adrenal origin". Physiological Research. 69 (Suppl 2): S187–S192. doi:10.33549/physiolres.934516. PMC 8603739. PMID 33094617.
  24. Freel EM, Shakerdi LA, Friel EC, Wallace AM, Davies E, Fraser R, Connell JM (September 2004). "Studies on the origin of circulating 18-hydroxycortisol and 18-oxocortisol in normal human subjects". The Journal of Clinical Endocrinology and Metabolism. 89 (9): 4628–33. doi:10.1210/jc.2004-0379. PMC 1283128. PMID 15356073.
  25. Nicod J, Dick B, Frey FJ, Ferrari P (February 2004). "Mutation analysis of CYP11B1 and CYP11B2 in patients with increased 18-hydroxycortisol production". Molecular and Cellular Endocrinology. 214 (1–2): 167–74. doi:10.1016/j.mce.2003.10.056. PMID 15062555. S2CID 617448.
  26. Lenders JW, Williams TA, Reincke M, Gomez-Sanchez CE (January 2018). "DIAGNOSIS OF ENDOCRINE DISEASE: 18-Oxocortisol and 18-hydroxycortisol: is there clinical utility of these steroids?". European Journal of Endocrinology. 178 (1): R1–R9. doi:10.1530/EJE-17-0563. PMC 5705277. PMID 28904009.
  27. Hanukoglu I, Privalle CT, Jefcoate CR (May 1981). "Mechanisms of ionic activation of adrenal mitochondrial cytochromes P-450scc and P-45011 beta" (PDF). J. Biol. Chem. 256 (9): 4329–35. doi:10.1016/S0021-9258(19)69437-8. PMID 6783659.
  28. Rapoport R, Sklan D, Hanukoglu I (March 1995). "Electron leakage from the adrenal cortex mitochondrial P450scc and P450c11 systems: NADPH and steroid dependence". Arch. Biochem. Biophys. 317 (2): 412–6. doi:10.1006/abbi.1995.1182. PMID 7893157.
  29. Hanukoglu I, Feuchtwanger R, Hanukoglu A (November 1990). "Mechanism of corticotropin and cAMP induction of mitochondrial cytochrome P450 system enzymes in adrenal cortex cells" (PDF). J. Biol. Chem. 265 (33): 20602–8. doi:10.1016/S0021-9258(17)30545-8. PMID 2173715.
  30. Miller WL (2019). "Congenital Adrenal Hyperplasia: Time to Replace 17OHP with 21-Deoxycortisol". Hormone Research in Paediatrics. 91 (6): 416–420. doi:10.1159/000501396. PMID 31450227. S2CID 201733086.

Further reading

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