Buffered charcoal yeast extract agar
Buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or grow certain types of bacteria, particularly the Gram-negative species Legionella pneumophila.[1] It has also been used for the laboratory diagnosis of Acanthamoeba keratitis,[2] Francisella and Nocardia.
| Ingredient | Amount (g/L) | Purpose |
|---|---|---|
| Agar | 12.0 | Solidifying |
| Yeast extract | 10.0 | Vitamins, nitrogen, and carbon |
| ACES buffer | 10.0 | Buffer |
| Activated charcoal | 2.0 | Decomposes hydrogen peroxide, a toxic metabolite of Legionella |
| Potassium hydroxide | 2.8 | pH (target shall be 6.9±0.2) |
| Alpha-ketoglutarate | 1.0 | Stimulates oxygen-scavenging enzymes in Legionella |
| L-cysteine | 0.4 | Provide absolutely essential nutrients to Legionella |
| Ferric pyrophosphate | 0.2 |
This culture is to be differentiated from regular yeast extract agar, which is not selective.
References
- "Archived copy" (PDF). Archived from the original (PDF) on 2012-06-12. Retrieved 2012-05-11.
{{cite web}}: CS1 maint: archived copy as title (link) - Penland RL, Wilhelmus KR (October 1998). "Laboratory diagnosis of Acanthamoeba keratitis using buffered charcoal-yeast extract agar". Am. J. Ophthalmol. 126 (4): 590–2. doi:10.1016/S0002-9394(98)00125-1. PMID 9780107.
- Aryal, Sagar (4 January 2022). "BCYE Agar- Composition, Principle, Preparation, Results, Uses". Microbe Notes.
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