XLT agar

XLT Agar (Xylose Lysine Tergitol-4) is a selective culture medium for the isolation and identification of salmonellae from food and environmental samples. It is similar to XLD agar; however, the agar is supplemented with the surfactant, Tergitol 4, which causes inhibition of Proteus spp. and other non-Salmonellae.[1] Successful growth of Salmonella will result in growth of red colonies with a black centre.

  • XLT Agar contains:
Proteose Peptone 1.6g/l
Yeast extract 3g/l
L-Lysine 5g/l
Xylose 3.75g/l
Lactose 7.5g/l
Sucrose 7.5g/l
Sodium chloride 5g/l
Sodium thiosulphate 6.8g/l
Ferric ammonium citrate 0.8 mg/l
Phenol red 0.08g/l
Agar 18g/l
Tergitol-4 4.6mL/l

See also

References

  1. Dusch, H. & Altwegg, M. (1995). "Evaluation of Five New Plating Media for Isolation of Salmonella Species". Journal of Clinical Microbiology. 33 (4): 802–804. doi:10.1128/jcm.33.4.802-804.1995. PMC 228044. PMID 7790441.


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