XLT agar
XLT Agar (Xylose Lysine Tergitol-4) is a selective culture medium for the isolation and identification of salmonellae from food and environmental samples. It is similar to XLD agar; however, the agar is supplemented with the surfactant, Tergitol 4, which causes inhibition of Proteus spp. and other non-Salmonellae.[1] Successful growth of Salmonella will result in growth of red colonies with a black centre.
- XLT Agar contains:
| Proteose Peptone | 1.6g/l |
| Yeast extract | 3g/l |
| L-Lysine | 5g/l |
| Xylose | 3.75g/l |
| Lactose | 7.5g/l |
| Sucrose | 7.5g/l |
| Sodium chloride | 5g/l |
| Sodium thiosulphate | 6.8g/l |
| Ferric ammonium citrate | 0.8 mg/l |
| Phenol red | 0.08g/l |
| Agar | 18g/l |
| Tergitol-4 | 4.6mL/l |
See also
- Agar plate
- XLD agar
- R2a agar
- MRS agar
References
- Dusch, H. & Altwegg, M. (1995). "Evaluation of Five New Plating Media for Isolation of Salmonella Species". Journal of Clinical Microbiology. 33 (4): 802–804. doi:10.1128/jcm.33.4.802-804.1995. PMC 228044. PMID 7790441.
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