Buffered charcoal yeast extract agar

Buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or grow certain types of bacteria, particularly the Gram-negative species Legionella pneumophila.[1] It has also been used for the laboratory diagnosis of Acanthamoeba keratitis,[2] Francisella and Nocardia.

Composition of BCYE agar medium[3]
IngredientAmount (g/L)Purpose
Agar12.0Solidifying
Yeast extract10.0Vitamins, nitrogen, and carbon
ACES buffer10.0Buffer
Activated charcoal2.0Decomposes hydrogen peroxide, a toxic metabolite of Legionella
Potassium hydroxide2.8pH (target shall be 6.9±0.2)
Alpha-ketoglutarate1.0Stimulates oxygen-scavenging enzymes in Legionella
L-cysteine0.4 Provide absolutely essential nutrients to Legionella
Ferric pyrophosphate0.2

This culture is to be differentiated from regular yeast extract agar, which is not selective.

References

  1. "Archived copy" (PDF). Archived from the original (PDF) on 2012-06-12. Retrieved 2012-05-11.{{cite web}}: CS1 maint: archived copy as title (link)
  2. Penland RL, Wilhelmus KR (October 1998). "Laboratory diagnosis of Acanthamoeba keratitis using buffered charcoal-yeast extract agar". Am. J. Ophthalmol. 126 (4): 590–2. doi:10.1016/S0002-9394(98)00125-1. PMID 9780107.
  3. Aryal, Sagar (4 January 2022). "BCYE Agar- Composition, Principle, Preparation, Results, Uses". Microbe Notes.
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