Overall hemostatic potential

Overall hemostatic potential
SynonymsOverall hemostasis potential; Overall haemostasis potential; Overall haemostatic potential; OHP
Test ofCoagulation, hypercoagulability, hypocoagulability, hemostasis, fibrinolysis

The overall hemostatic potential (OHP) test is a global coagulation assay which can be used to measure coagulation.[1][2][3][4] The OHP assay measures total fibrin generation in the presence of thrombin or tissue factor and tissue plasminogen activator (t-PA).[2][1][3][4] It generates a fibrin time curve through the use of optical density measurement.[1] This curve represents the balance between fibrin formation induced by thrombin or tissue factor and fibrinolysis induced by t-PA.[1] The assay provides three parameters: overall coagulation potential (OCP), overall hemostatic potential (OHP), and overall fibrinolytic potential (OFP).[2][1][4] OHP is the main parameter, while OCP and OFP are supplementary parameters to assess coagulation and fibrinolysis.[3] One further parameter, clot lysis time (CLT), can also be determined.[3] The OHP assay measures the integrated effect of procoagulant, anticoagulant, and fibrinolytic factors.[1]

The OHP is a technically simple[1][3] but relatively labor-intensive assay.[2] As of 2010, it had been implemented in several laboratories, but was not available commercially.[3] The assay is novel in terms of its combined evaluation of both fibrin generation and fibrinolysis.[1] The test is potentially useful in the evaluation of hypercoagulability, hypocoagulability, and fibrinolytic abnormalities.[1][2][3] It is able to detect hypercoagulability associated with pregnancy and estrogen/progestogen hormone therapy.[3][4] The test has also been used to study coagulation in feminizing hormone therapy in transgender women.[5] However, more studies are needed to validate the test as a biomarker of thrombosis and other abnormalities of coagulation.[2][3][4]

History

The original OHP assay was developed in Sweden by Blombäck and colleagues and was first described in 1999.[1][6][7] It used thrombin to trigger coagulation.[1] Since then, a modified version has been developed which can use either thrombin or tissue factor to trigger coagulation.[1][3][8][9]

References

  1. 1 2 3 4 5 6 7 8 9 10 11 12 Curnow J (2017). "The Overall Hemostatic Potential (OHP) Assay". Hemostasis and Thrombosis. Methods Mol Biol. Vol. 1646. pp. 523–531. doi:10.1007/978-1-4939-7196-1_38. ISBN 978-1-4939-7194-7. PMID 28804852.
  2. 1 2 3 4 5 6 Lim HY, Donnan G, Nandurkar H, Ho P (January 2022). "Global coagulation assays in hypercoagulable states". J Thromb Thrombolysis. 54 (1): 132–144. doi:10.1007/s11239-021-02621-1. PMID 34997471. S2CID 245804095.
  3. 1 2 3 4 5 6 7 8 9 10 Antovic A (October 2010). "The overall hemostasis potential: a laboratory tool for the investigation of global hemostasis". Semin Thromb Hemost. 36 (7): 772–9. doi:10.1055/s-0030-1265294. PMID 20978998.
  4. 1 2 3 4 5 Antovic A (July 2008). "Screening haemostasis--looking for global assays: the Overall Haemostasis Potential (OHP) method--a possible tool for laboratory investigation of global haemostasis in both hypo- and hypercoagulable conditions". Curr Vasc Pharmacol. 6 (3): 173–85. doi:10.2174/157016108784912028. PMID 18673157.
  5. ↑ Lim HY, Leemaqz SY, Torkamani N, Grossmann M, Zajac JD, Nandurkar H, Ho P, Cheung AS (July 2020). "Global Coagulation Assays in Transgender Women on Oral and Transdermal Estradiol Therapy". J Clin Endocrinol Metab. 105 (7): e2369–e2377. doi:10.1210/clinem/dgaa262. PMID 32413907.
  6. ↑ He S, Bremme K, Blombäck M (October 1999). "A laboratory method for determination of overall haemostatic potential in plasma. I. Method design and preliminary results". Thromb Res. 96 (2): 145–56. doi:10.1016/s0049-3848(99)00092-4. PMID 10574592.
  7. ↑ He S, Antovic A, Blombäck M (September 2001). "A simple and rapid laboratory method for determination of haemostasis potential in plasma. II. Modifications for use in routine laboratories and research work". Thromb Res. 103 (5): 355–61. doi:10.1016/s0049-3848(01)00332-2. PMID 11553368.
  8. ↑ Curnow JL, Morel-Kopp MC, Roddie C, Aboud M, Ward CM (March 2007). "Reduced fibrinolysis and increased fibrin generation can be detected in hypercoagulable patients using the overall hemostatic potential assay". J Thromb Haemost. 5 (3): 528–34. doi:10.1111/j.1538-7836.2007.02362.x. PMID 17166248. S2CID 25346726.
  9. ↑ Curnow, J. L. (2010). Evaluation of the overall haemostatic potential assay for the diagnosis and management of hypercoagulable states (Doctoral dissertation, University of Sydney). https://scholar.google.com/scholar?cluster=17586055299192023070
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