Aphthovirus

Aphthovirus
Electronmicrograph of "Foot-and-mouth disease virus"
Electronmicrograph of Foot-and-mouth disease virus
Virus classification
(unranked): Virus
Realm: Riboviria
Kingdom: Orthornavirae
Phylum: Pisuviricota
Class: Pisoniviricetes
Order: Picornavirales
Family: Picornaviridae
Genus: Aphthovirus
Species
  • Bovine rhinitis A virus
  • Bovine rhinitis B virus
  • Equine rhinitis A virus
  • Foot-and-mouth disease virus

Aphthovirus (from the Greek aphtha-, vesicles in the mouth) is a viral genus of the family Picornaviridae. Aphthoviruses infect split-hooved animals, and include the causative agent of foot-and-mouth disease, Foot-and-mouth disease virus (FMDV).[1] There are seven FMDV serotypes: A, O, C, SAT 1, SAT 2, SAT 3 and Asia 1, and four non-FMDV serotypes belonging to three additional species Bovine rhinitis A virus (BRAV), Bovine rhinitis B virus (BRBV) and Equine rhinitis A virus (ERAV).

Structure

Genome and structure of foot-and-mouth disease virus

Aphthoviruses are non-enveloped and have an icosahedral capsid with a diameter of around 27 to 30 nm.[2] The assembled viral capsid contains a single copy of the RNA genome and 60 copies of the four viral capsid proteins VP1, VP2, VP3, and VP4. The protomers form the sides of the icosahedral capsid. The VP4 protein is internal.[3]

GenusStructureSymmetryCapsidGenomic arrangementGenomic segmentation
AphthovirusIcosahedralPseudo T=3Non-envelopedLinearMonopartite

Genome

The aphthoviruses are differentiated from other picornaviruses as they have a larger genome (7.5–8.5 kilobases).[2] The genome is non-segmented and consists of a positive-sense single-stranded RNA. It contains a single open reading frame with a 5' end linked protein (VPg), which is associated with the genome via a phosphodiester bond linked to a tyrosine residue.[3] The 5' untranslated region (UTR) of the genome contains a poly(C) tract and an internal ribosome entry site (IRES), while the 3' UTR is polyadenylated.[4] The P1 region encodes the structural proteins. The P2 and P3 regions encode the nonstructural proteins associated with replication.[3]

Replication

Replication cycle of Foot-and-mouth disease virus

Aphthoviruses replicate in a similar fashion to all picornaviruses. Replication is cytoplasmic and initially involves attachment of the exogenous virus to the cell membrane. Attachment to the membrane and subsequent entry into the cell is mediated by a membrane receptor. After genome replication within the cytoplasm, virion assembly occurs and new virus particles aggregate within the cell. Release of virus particles is mediated by cell lysis.[1]

Recombination

The aphthovirus RNA genome is able to undergo genetic recombination.[5] Recombination occurs at a large number of genomic sites indicating that RNA recombination in aphthovirus is a general, rather than a site specific, phenomenon.[6]

Pathology and ecology

Aphthoviruses include the causative agents of foot-and-mouth disease (FMD), which primarily affects livestock such as cattle, swine, sheep and goats. FMD was first discovered in Italy in the 16th century. Since then, the prevalence of the disease has remained, despite many countries being declared FMD-free. Endemic regions of the disease include areas of Africa, Asia and South America. The virus commonly persists in animal feed and is able to survive environmentally for up to one month. Eradication of FMD in endemic areas has been difficult, despite the availability of a vaccine.[1]

Equine rhinitis A virus (ERAV) was first isolated from horses in the 1960s and 1970s showing acute febrile respiratory disease signs, including fever, cough, clear nasal discharge and lethargy. Given its similarity to the common cold in humans (caused by another picornavirus, rhinovirus), ERAV was initially named "equine rhinovirus 1". Modern molecular biology techniques such as nucleotide sequencing demonstrated that ERAV was in fact more closely related to FMDV, and was reclassified to the genus Aphthovirus.[7]

Genus[2]Host detailsTissue tropismEntry detailsRelease detailsReplication siteAssembly siteTransmission
AphthovirusRuminants (i.e. cattle, bison, sheep), pigs, horses.Epithelium: soft palate; epithelium: pharynx; epithelium: lung; epithelium: feet; epithelium: mouthClathrin-mediated endocytosisLysisCytoplasmCytoplasmContact; saliva; aerosol

See also

  • Animal virology

References

  1. 1 2 3 Martinez-Salas; et al. (2008). "Foot-and-Mouth Disease Virus". Animal Viruses: Molecular Biology. Caister Academic Press. ISBN 978-1-904455-22-6.
  2. 1 2 3 "Viral Zone". ExPASy. Retrieved 12 June 2015.
  3. 1 2 3 Jamal, Syed M.; Belsham, Graham J. (2013-12-05). "Foot-and-mouth disease: past, present and future". Veterinary Research. 44 (1): 116. doi:10.1186/1297-9716-44-116. ISSN 1297-9716. PMC 4028749. PMID 24308718.
  4. ↑ Büchen-Osmond, C. (Ed). "00.052.0.05. Aphthovirus". ICTVdB - The Universal Virus Database, version 4. New York, USA: Columbia University. Archived from the original on December 3, 2008.
  5. ↑ King, A. M.; McCahon, D.; Slade, W. R.; Newman, J. W. (1982). "Biochemical evidence of recombination within the unsegmented RNA genome of aphthovirus". Journal of Virology. 41 (1): 66–77. doi:10.1128/JVI.41.1.66-77.1982. PMC 256726. PMID 6283129.
  6. ↑ King, Andrew M.Q.; McCahon, David; Saunders, Keith; Newman, John W.I.; Slade, William R. (1985). "Multiple sites of recombination within the RNA genome of foot-and-mouth disease virus". Virus Research. 3 (4): 373–384. doi:10.1016/0168-1702(85)90437-x. PMC 7134178. PMID 3000107.
  7. ↑ Wutz G, Auer H, Nowotny N, Grosse B, Skern T, Kuechler E (1996). "Equine rhinovirus serotypes 1 and 2: relationship to each other and to aphthoviruses and cardioviruses". J Gen Virol. 77 ( Pt 8) (8): 1719–30. doi:10.1099/0022-1317-77-8-1719. PMID 8760418.
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