Coltivirus

Taï Forest coltivirus (white arrows) with inner and outer icosahedral capsids of around 70 nm in diameter

The Coltivirus virions are about 60-80 nanometers in diameter and are not enveloped, and are generally a spherical shape with icosahedral symmetry. Each virion has two concentric capsid shells surrounding a core of about 50 nanometers in diameter. The surface of the particle is relatively smooth.[7] The virus loses its infectivity when the surrounding fluid becomes acidic, around a pH of three, but is stable when the pH is between seven and eight. It also stops being a threat when the temperature becomes about fifty-five degrees Celsius.[7]

Genome map of species Colorado tick fever virus (CTFV) showing several segments.[1]

Coltiviruses have twelve segments of linear, double-stranded RNA. When the genome is processed with gel electrophoresis, the segments migrate as three class sizes (three bands). Each segment is replicated, and the largest segment encodes RNA polymerase.[7] Locations of other proteins along the genome have yet to be determined.

Red blood cells are the target of Colorado tick fever virus

Coltiviruses replicate in the cytoplasm in cells of both arthropods and vertebrates, but they are only transmitted by the arthropods.[5] When the virus replicates, the virion outer shell has to be removed in order for RNA polymerase to be activated to continue the replication of the virion's RNA. Reassortment of the RNA segments in progeny is common, and this plays a role in some of the genetic diversity between the serotypes. Sometimes, this can lead to fast changes in the properties of the viruses.[5]

The main species of Coltivirus, Colorado tick fever virus (CTF), infects the Rocky Mountain wood tick (Dermacentor andersoni). This species of tick can be found in areas with shrubs, lightly wooded locations, grasslands, and on hiking or biking trails. All life stages of this tick can infect vertebrates with Colorado tick fever virus (larva, nymph, and adult). Unfortunately, mature D. andersoni prefer to feed on medium or large mammals that are walking around the knee-high plants.[9] Oftentimes, this infection takes place when the tick larvae feed on rodents, like squirrels, that are already infected with virus. The tick's saliva then contains the virus, and it becomes infectious for life. The adult tick then transmits the virus to humans through a bite, where it infects bone marrow cells.[5]

The virus replicates in those bone marrow cells, which disrupts the development and replication of leukocytes (white blood cells), eosinophils, and basophils. Because of this, thrombocytopenia could also a potential result. Erythrocytes, which are enucleated red blood cells, seem to be infected while they are erythroblasts, their nucleated precursor stage.[5] The virus stays in these red blood cells without harming it for up to four months.[7] Here, it is protected from the immune system's attacks.[3] Antibody to the virus is found only about two weeks after symptoms begin to show, but the virus can still be found in blood cells for about six weeks.[5]

Colorado tick fever virus can cause a fever, chills, headache, photophobia, myalgia, arthralgia, and lethargy. Children, in particular, may develop a hemorrhagic disease. Leukopenia with both lymphocytes and neutrophils is very common for Colorado tick fever virus. In either case, the infection can lead to encephalitis or meningitis.[3]

For diagnosis, the erythrocytes can be isolated by injecting them into a tissue culture and checking to see if they are infected. Also, the antigen for Colorado tick fever virus can be identified using the immunofluorescence microscopy. In this method, the antigens on the surface of the erythrocytes are marked with fluorescence and examined under a fluorescence microscope.[5]

Range map of Dermacentor andersoni in the United States

Brown shading represents the Rocky Mountain Range

The distribution of Colorado tick fever virus is in the Rocky Mountain area of the United States at elevations between four and ten thousand feet.[7] Not surprisingly, Colorado tick fever virus can be found in places like California, Colorado, Idaho, Montana, Nevada, Oregon, Utah, Washington, Wyoming, British Columbia, and Alberta. This is roughly the same distribution as the tick that transmits the virus, shown in the adjacent picture.[5]

The virus circulates between ticks and rodents, with humans being the secondary hosts.[5] People at risk for catching the disease are hikers and campers that are in the risk areas. Also, April, May, and June are when the infections mainly occur, because this is the time when the adult ticks are prevalent in the environment. Unfortunately, this is also when the weather is pleasant for hiking and camping. The best way to avoid getting bitten and catching this disease is wearing long sleeves or pants, avoiding high tick-infested areas, and wearing tick repellent.[5]

Reverse transcription-polymerase chain reaction (RT-PCR) procedure

Colorado tick fever virus can be detected in a patient with a reverse transcription polymerase chain reaction (RT-PCR), where even a single virion and its genetic material can be detected.[7] The antigens to the virus can also be detected using immunofluorescence.[5] There is currently no known vaccine or treatment available to treat these Coltiviruses, but 3'-fluoro-3'-deoxyadenosine, a nucleoside analog, halts replication of Colorado tick fever virus in vitro.[5]