Potyviridae

Potyviridae is a family of positive-strand RNA viruses that encompasses more than 30% of known plant viruses,[1][2] many of which are of great agricultural significance. The family has 12 genera and 235 species, three of which are unassigned to a genus.[3][4][5][6]

Potyviridae
Genome of plum pox virus of genus Potyvirus with transmission electron micrograph and model of virions
Virus classification
(unranked): Virus
Realm: Riboviria
Kingdom: Orthornavirae
Phylum: Pisuviricota
Class: Stelpaviricetes
Order: Patatavirales
Family: Potyviridae
Genera

see text

Structure
Genomic map of a typical member of the genus Potyvirus.

Potyvirid virions are nonenveloped, flexuous filamentous, rod-shaped particles. The diameter is around 12–15 nm, with a length of 200–300 nm.[4][5]

Genome

Genomes are linear and usually nonsegmented, around 8–12kb in length,[4][5] consisting of positive-sense RNA, which is surrounded by a protein coat made up of a single viral encoded protein called a capsid. All induce the formation of virus inclusion bodies called cylindrical inclusions (‘pinwheels’) in their hosts. These consist of a single protein (about 70 kDa) made in their hosts from a single viral genome product.

Member viruses encode large polypeptides that are cleaved into mature proteins. In 5'–3' order these proteins are

  • P1 (a serine protease): 83 kDa
  • HC (a protease): 51 kDa
  • P3: 34 kDa
  • 6K1: 5 kDa
  • Cl (helicase): 71 kDa
  • 6K2: 6 kDa
  • VPg (the 5' binding protein): 20 kDa
  • NIa-Pro (a protease): 27 kDa
  • NIb (RNA dependent RNA polymerase): 57 kDa
  • Capsid protein: 34 kDa

There may be some variation in the number of the proteins depending on the genera and species.[7] For instance some genera lack P1, some virus of the genus Ipomovirus lack HC and have a P1 tandem. Pretty interesting sweet potato potyviral ORF (PISPO), alkylation B (AlkB), and inosine triphosphate pyrophosphatase (known as ITPase or HAM1) are protein domains identified in atypical members.[7]

Life cycle
Replication and movement of soybean mosaic virus (SMV)

Viral replication is cytoplasmic. Entry into the host cell is achieved by penetration. Replication follows the positive-stranded RNA virus replication model. Positive-stranded RNA virus transcription is the method of transcription. Translation takes place by −1 ribosomal frameshifting. The virus exits the host cell by tubule-guided viral movement.[4][5] Plants serve as the natural host. The virus is transmitted via a vector (often an insect or mite). Transmission routes are vector and mechanical.[4][5]

Transmission

Potyvirus is the largest genus in the family, with 183 known species.[8] These viruses are 720–850 nm in length and are transmitted by aphids. They can also be easily transmitted by mechanical means. These viruses shared a common ancestry 6,600 years ago[9] and are transmitted by over 200 species of aphids.

The species in the genus Macluravirus are 650–675 nm in length and are also transmitted by aphids. The plant viruses in the genus Ipomovirus are transmitted by whiteflies and they are 750–950 nm long. Tritimovirus and the Rymovirus viruses are 680–750 nm long and are transmitted by eriophydid mites. (The rymoviruses are closely related to the potyviruses and may eventually be merged with the potyviruses.[10]) The Bymovirus genome consists of two particles instead of one (275 and 550 nm) and these viruses are transmitted by the chytrid fungus, Polymyxa graminis.

Taxonomy
Phylogenetic tree of family Potyviridae

The following genera are recognized:[6]

The following species are unassigned to a genus:[6]

References
  1. Riechmann, JL; Lain, S; Garcia, JA (1992). "Highlights and prospects of potyvirus molecularbiology". J Gen Virol. 73: 1–16. doi:10.1099/0022-1317-73-1-1. PMID 1730931.
  2. Berger PH, et al. (2005) in Virus Taxonomy: Eighth Report of the InternationalCommittee on the Taxonomy of Viruses, eds Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (Elsevier Academic, San Diego), pp 819–841.
  3. Wylie, SJ; Adams, M; Chalam, C; Kreuze, J; López-Moya, JJ; Ohshima, K; Praveen, S; Rabenstein, F; Stenger, D; Wang, A; Zerbini, FM; ICTV Report Consortium (March 2017). "ICTV Virus Taxonomy Profile: Potyviridae". The Journal of General Virology. 98 (3): 352–354. doi:10.1099/jgv.0.000740. PMC 5797945. PMID 28366187.
  4. "Potyviridae". ICTV Online (10th) Report.
  5. "Viral Zone". ExPASy. Retrieved 15 June 2015.
  6. "Virus Taxonomy: 2020 Release". International Committee on Taxonomy of Viruses (ICTV). March 2021. Retrieved 21 May 2021.
  7. Pasin, Fabio; Daròs, José-Antonio; Tzanetakis, Ioannis E (2022). "Proteome expansion in the Potyviridae evolutionary radiation". FEMS Microbiology Reviews: fuac011. doi:10.1093/femsre/fuac011. ISSN 1574-6976. PMID 35195244.
  8. Description of Plant Viruses: Family Potyviridae
  9. Nigam D, LaTourrette K, Souza PFN, Garcia-Ruiz H (2019) Genome-wide variation in Potyviruses. Front Plant Sci 10:1439
  10. Description of Plant Viruses: Family Potyviridae figure
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