Phytoreovirus

Phytoreovirus is a genus of viruses, in the family Reoviridae, in the subfamily Sedoreovirinae. They are non-turreted reoviruses that are major agricultural pathogens, particularly in Asia.[1] Oryza sativa for RDV and RGDV, dicotyledonous for WTV, and leafhoppers serve as natural hosts. There are three species in this genus. Diseases associated with this genus include: WTV: galls (tumor). RDV: dwarf (or stunt) disease of rice. RGDV: dwarfing, stunting, and galls.[2][3]

Phytoreovirus
Cryo-EM of protein capsid structure of rice dwarf virus (RDV)
Virus classification
(unranked): Virus
Realm: Riboviria
Kingdom: Orthornavirae
Phylum: Duplornaviricota
Class: Resentoviricetes
Order: Reovirales
Family: Reoviridae
Subfamily: Sedoreovirinae
Genus: Phytoreovirus

Classification

One member of this family, Rice Dwarf Virus (RDV), has been extensively studied by electron cryomicroscopy and x-ray crystallography. From these analyses, atomic models of the capsid proteins and a plausible model for capsid assembly have been derived. While the structural proteins of RDV share no sequence similarity to other proteins, their folds and the overall capsid structure are similar to those of other reoviruses.[1]

Phytoreoviruses are unique among reoviruses in that there are 12 dsRNA segments in each virus. Like all other reoviruses, they contain their own RNA-dependent RNA polymerase, allowing for endogenous synthesis of viral mRNA within the virus. Although less well studied than orthoreovirus and orbivirus, considerable structural and biochemical studies have been undertaken to characterize phytoreoviruses, which in general infect plants. There are three recognized phytoreoviruses: Wound tumour virus (WTV), Rice dwarf virus (RDV) and Rice gall dwarf virus (RGDV). Possible additional family members include tobacco leaf enation virus (TLEF), rice bunchy stunt virus and sweet potato virus (ICTV, VIDE). There are also several RDV isolates, all of which share over 90% sequence identity. Of these phytoreoviruses, RDV has been extensively studied and is the best characterized member. Most of our knowledge about phytoreovirus assembly and structure is based on structural data accumulated in RDV studies.[1]

Taxonomy

The following three species are assigned to the genus:[3]

  • Rice dwarf virus
  • Rice gall dwarf virus
  • Wound tumor virus

Structure

Viruses in Phytoreovirus are non-enveloped, with icosahedral geometries, and T=13, T=2 symmetry. The diameter is around 70 nm. Genomes are linear and segmented. Segments range in size from 1066 to 4423 base pairs, resulting in a total genome length of around 26kb. The genome codes for 15 proteins and has 4 open reading frames.[2]

GenusStructureSymmetryCapsidGenomic arrangementGenomic segmentation
PhytoreovirusIcosahedralT=13, T=2Non-envelopedLinearSegmented

Life cycle

Viral replication is cytoplasmic. Entry into the host cell is achieved by penetration into the host cell. Replication follows the double-stranded RNA virus replication model. Double-stranded RNA virus transcription is the method of transcription. The virus exits the host cell by monopartite non-tubule guided viral movement. Oryza sativa for rdv and rgdv and dicotyledonous for wtv serve as the natural host. The virus is transmitted via a vector (insect mechanical inoculation in plant by leafhoppers). Transmission routes are vector and mechanical.[2]

GenusHost detailsTissue tropismEntry detailsRelease detailsReplication siteAssembly siteTransmission
PhytoreovirusOryza sativa; leafhoppersPhloemViral movement; mechanical inoculationCell deathCytoplasmCytoplasmLeafhoppers

See also

References
  1. Baker; et al. (2008). "Structures of Phytoreoviruses". Segmented Double-stranded RNA Viruses: Structure and Molecular Biology. Caister Academic Press. ISBN 978-1-904455-21-9.
  2. "Viral Zone". ExPASy. Retrieved 15 June 2015.
  3. "Virus Taxonomy: 2020 Release". International Committee on Taxonomy of Viruses (ICTV). March 2021. Retrieved 13 May 2021.
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