Tre recombinase

Tre recombinase is an experimental enzyme that in lab tests has removed DNA inserted by HIV from infected cells.[1] Through selective mutation, Cre recombinase which recognizes loxP sites are modified to identify HIV long terminal repeats (loxLTR) instead. As a result, instead of performing Cre-Lox recombination, the new enzyme performs recombination at HIV provirus sites.[2]

The structure of Tre in complex with loxLTR has been resolved (PDB: 5U91), allowing for analyzing the roles of individual mutations.[3]

References

Sarkar, Indrani; Hauber, Ilona; Hauber, Joachim; Buchholz, Frank (2007). "HIV-1 proviral DNA excision using an evolved recombinase". Science. 316 (5833): 1912–15. Bibcode:2007Sci...316.1912S. doi:10.1126/science.1141453. PMID 17600219. S2CID 2437602.
Hauber, Ilona; Hofmann-Sieber, Helga; Chemnitz, Jan; et al. (September 26, 2013). "Highly Significant Antiviral Activity of HIV-1 LTR-Specific Tre-Recombinase in Humanized Mice". PLOS Pathogens. 9 (9): e1003587. doi:10.1371/journal.ppat.1003587. PMC 3784474. PMID 24086129.
Meinke, G; Karpinski, J; Buchholz, F; Bohm, A (19 September 2017). "Crystal structure of an engineered, HIV-specific recombinase for removal of integrated proviral DNA". Nucleic Acids Research. 45 (16): 9726–9740. doi:10.1093/nar/gkx603. PMC 5766204. PMID 28934476.

External links

Minkel, JR (June 28, 2007). "Designer enzyme cuts HIV out of infected cells". Scientific American.

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[pmid17600219-1] Sarkar, Indrani; Hauber, Ilona; Hauber, Joachim; Buchholz, Frank (2007). "HIV-1 proviral DNA excision using an evolved recombinase". Science. 316 (5833): 1912–15. Bibcode:2007Sci...316.1912S. doi:10.1126/science.1141453. PMID 17600219. S2CID 2437602.

[2] Hauber, Ilona; Hofmann-Sieber, Helga; Chemnitz, Jan; et al. (September 26, 2013). "Highly Significant Antiviral Activity of HIV-1 LTR-Specific Tre-Recombinase in Humanized Mice". PLOS Pathogens. 9 (9): e1003587. doi:10.1371/journal.ppat.1003587. PMC 3784474. PMID 24086129.

[3] Meinke, G; Karpinski, J; Buchholz, F; Bohm, A (19 September 2017). "Crystal structure of an engineered, HIV-specific recombinase for removal of integrated proviral DNA". Nucleic Acids Research. 45 (16): 9726–9740. doi:10.1093/nar/gkx603. PMC 5766204. PMID 28934476.