Yersinia pestis


A scanning electron micrograph depicting a mass of Yersinia pestis bacteria in the foregut of an infected flea

Scientific Classification

Domain: Bacteria

Phylum: Pseudomonadota

Class: Gammaproteobacteria

Order: Enterobacterales

Family: Yersiniaceae

Genus: Yersinia

Species: Y. pestis

Yersinia pestis (Y. pestis; formerly Pasteurella pestis) is a gram-negative, non-motile, coccobacillus bacterium without spores that is related to both Yersinia pseudotuberculosis and Yersinia enterocolitica. It is a facultative anaerobic organism that can infect humans via the Oriental rat flea (Xenopsylla cheopis).[1] It causes the disease plague, which caused the first plague pandemic and the Black Death, the deadliest pandemic in recorded history. Plague takes three main forms: pneumonic, septicemic, and bubonic. Yersinia pestis is a parasite of its host, the rat flea, which is also a parasite of rats, hence Y. pestis is a hyperparasite.

Y. pestis was discovered in 1894 by Alexandre Yersin, a Swiss/French physician and bacteriologist from the Pasteur Institute, during an epidemic of the plague in Hong Kong.[2][3] Yersin was a member of the Pasteur school of thought. Kitasato Shibasaburō, a Japanese bacteriologist who practised Koch's methodology, was also engaged at the time in finding the causative agent of the plague.[4] However, Yersin actually linked plague with a bacillus, initially named Pasteurella pestis; it was renamed Yersinia pestis in 1944.[5]

Every year, between one thousand and two thousand cases of the plague are still reported to the World Health Organization.[6] With proper antibiotic treatment, the prognosis for victims is much better than before antibiotics were developed. A five- to six-fold increase in cases occurred in Asia during the time of the Vietnam War, possibly due to the disruption of ecosystems and closer proximity between people and animals. The plague is now commonly found in sub-Saharan Africa and Madagascar, areas that now account for over 95% of reported cases. The plague also has a detrimental effect on non-human mammals;[7] in the United States, these include the black-tailed prairie dog and the endangered black-footed ferret.

A 2022 paper indicates that the long thought rat may not have been what caused the spread of the Black death.[8]

Bacterial features

Y. pestis is a non-motile coccobacillus, a facultative anaerobic bacterium with bipolar staining (giving it a safety pin appearance) that produces an antiphagocytic slime layer.[9] Similar to other Yersinia species, it tests negative for urease, lactose fermentation, and indole.[10] Its closest relatives are the gastrointestinal pathogen Yersinia pseudotuberculosis, and, more distantly, Yersinia enterocolitica.[11]

  • Yersinia pestis fluorescent
    Yersinia pestis fluorescent
  • SEM of Yersinia pestis
    SEM of Yersinia pestis
  • Yersinia pestis
    Yersinia pestis

Genome and proteome

Genome

Several complete genome sequences are available for various strains and subspecies of Y. pestis: strain KIM (of biovar Y. p. medievalis),[12] and strain CO92 (of biovar Y. p. orientalis, obtained from a clinical isolate in the United States).[13] In 2006 the genome sequence of a strain of biovar Antiqua was completed.[14] Some strains are non-pathogenic, such as that of strain 91001, whose sequence was published in 2004.[15]

Whole genome alignment of 8 Yersinia genomes via Mauve reveals 78 locally collinear blocks conserved among all 8 taxa
Features of Yersinia pestis genomes[15]
KIM CO92 91001
length (bp) 4,600,755 4,653,728 4,595,065
proteins encoded 4,198 4,012 4,037
pseudogenes 54 149 141
tRNAs 73 70 72

Plasmids

Like Y. pseudotuberculosis and Y. enterocolitica, Y. pestis is host to the plasmid pCD1. It also hosts two other plasmids, pPCP1 (also called pPla or pPst) and pMT1 (also called pFra) that are not carried by the other Yersinia species. pFra codes for a phospholipase D that is important for the ability of Y. pestis to be transmitted by fleas.[16] pPla codes for a protease, Pla, that activates plasmin in human hosts and is a very important virulence factor for pneumonic plague.[17] Together, these plasmids, and a pathogenicity island called HPI, encode several proteins that cause the pathogenesis for which Y. pestis is famous. Among other things, these virulence factors are required for bacterial adhesion and injection of proteins into the host cell, invasion of bacteria in the host cell, and acquisition and binding of iron harvested from red blood cells.[18][19]

Proteome

A comprehensive and comparative proteomics analysis of Y. pestis strain KIM was performed in 2006,[20] the analysis focused on the transition to a growth condition mimicking growth in host cells.

Small noncoding RNA

Numerous bacterial small noncoding RNAs have been identified to play regulatory functions. Some can regulate the virulence genes. Some 63 novel putative sRNAs were identified through deep sequencing of the Y. pestis sRNA-ome. Among them was Yersinia-specific (also present in Y. pseudotuberculosis and Y. enterocolitica) Ysr141 (Yersinia small RNA 141). Ysr141 sRNA was shown to regulate the synthesis of the type III secretion system (T3SS) effector protein YopJ.[21] The Yop-Ysc T3SS is a critical component of virulence for Yersinia species.[22] Many novel sRNAs were identified from Y. pestis grown in vitro and in the infected lungs of mice suggesting they play role in bacterial physiology or pathogenesis. Among them sR035 predicted to pair with SD region and transcription initiation site of a thermo-sensitive regulator ymoA, and sR084 predicted to pair with fur, ferric uptake regulator.[23]

Pathogenesis and transmission


In the urban and sylvatic cycles of Y. pestis, most of the spreading occurs between rodents and fleas. In the sylvatic cycle, the rodent is wild, but in the urban cycle, the rodent is primarily the brown rat, Rattus norvegicus. In addition, Y. pestis can spread from the urban environment and back. Transmission to humans is usually through the bite of infected fleas. If the disease has progressed to the pneumonic form, humans can spread the bacterium to others by coughing, vomiting, and possibly sneezing.[24][25][26]

Mammals as hosts

Rattus norvegicus/Brown rat

Several species of rodents serve as the main reservoir for Y. pestis in the environment. In the steppes, the natural reservoir is believed to be principally the marmot. In the western United States, several species of rodents are thought to maintain Y. pestis. However, the expected disease dynamics have not been found in any rodent. Several species of rodents are known to have a variable resistance, which could lead to an asymptomatic carrier status.[27] Evidence indicates fleas from other mammals have a role in human plague outbreaks.[28]

The lack of knowledge of the dynamics of plague in mammal species is also true among susceptible rodents such as the black-tailed prairie dog (Cynomys ludovicianus), in which plague can cause colony collapse, resulting in a massive effect on prairie food webs.[29] However, the transmission dynamics within prairie dogs do not follow the dynamics of blocked fleas; carcasses, unblocked fleas, or another vector could possibly be important, instead.[30]

The CO92 strain was isolated from a patient who died from pneumonia and who contracted the infection from an infected cat.[13]

In other parts of the world, the reservoir of the infection is not clearly identified, which complicates prevention and early-warning programs. One such example was seen in a 2003 outbreak in Algeria.[31]

Fleas as vector

Oriental rat flea (Xenopsylla cheopis) infected with the Y. pestis bacterium, which appears as a dark mass in the gut: The foregut (proventriculus) of this flea is blocked by a Y. pestis biofilm; when the flea attempts to feed on an uninfected host, Y. pestis is regurgitated into the wound, causing infection.

The transmission of Y. pestis by fleas is well characterized.[32] Initial acquisition of Y. pestis by the vector occurs during feeding on an infected animal. Several proteins then contribute to the maintenance of the bacteria in the flea digestive tract, among them the hemin storage system and Yersinia murine toxin (Ymt). Although Ymt is highly toxic to rodents and was once thought to be produced to ensure reinfection of new hosts, it is essential for flea colonization and for the survival of Y. pestis in fleas.[16][13]

The hemin storage system plays an important role in the transmission of Y. pestis back to a mammalian host.[33] While in the insect vector, proteins encoded by hemin storage system genetic loci induce biofilm formation in the proventriculus, a valve connecting the midgut to the esophagus.[34][35] The presence of this biofilm seems likely to be required for stable infection of the flea.[36] Aggregation in the biofilm inhibits feeding, as a mass of clotted blood and bacteria forms (referred to as "Bacot's block" after entomologist A.W. Bacot, the first to describe this phenomenon).[37] Transmission of Y. pestis occurs during the futile attempts of the flea to feed. Ingested blood is pumped into the esophagus, where it dislodges bacteria lodged in the proventriculus, which is regurgitated back into the host circulatory system.[37]

Humans and infection

Acral necrosis due to bubonic plague

Pathogenesis due to Y. pestis infection of mammalian hosts is due to several factors, including an ability of these bacteria to suppress and avoid normal immune system responses such as phagocytosis and antibody production. Flea bites allow for the bacteria to pass the skin barrier. Y. pestis expresses a plasmin activator that is an important virulence factor for pneumonic plague and that might degrade on blood clots to facilitate systematic invasion.[17] Many of the bacteria's virulence factors are antiphagocytic in nature. Two important antiphagocytic antigens, named F1 (fraction 1) and V or LcrV, are both important for virulence.[9] These antigens are produced by the bacterium at normal human body temperature. Furthermore, Y. pestis survives and produces F1 and V antigens while it is residing within white blood cells such as monocytes, but not in neutrophils. Natural or induced immunity is achieved by the production of specific opsonic antibodies against F1 and V antigens; antibodies against F1 and V induce phagocytosis by neutrophils.[38]

In addition, the type-III secretion system (T3SS) allows Y. pestis to inject proteins into macrophages and other immune cells. These T3SS-injected proteins, called Yersinia outer proteins (Yops), include Yop B/D, which form pores in the host cell membrane and have been linked to cytolysis. The YopO, YopH, YopM, YopT, YopJ, and YopE are injected into the cytoplasm of host cells by T3SS into the pore created in part by YopB and YopD;[39] the injected Yops limit phagocytosis and cell signaling pathways important in the innate immune system.

Y. pestis proliferates inside lymph nodes, where it is able to avoid destruction by cells of the immune system such as macrophages. The ability of Y. pestis to inhibit phagocytosis allows it to grow in lymph nodes and cause lymphadenopathy. YopH is a protein tyrosine phosphatase that contributes to the ability of Y. pestis to evade immune system cells.[40] In macrophages, YopH has been shown to dephosphorylate p130Cas and Pyk, a tyrosine kinase homologous to FAK. YopH also binds the p85 subunit of phosphoinositide 3-kinase, the Gab1, the Gab2 adapter proteins.[41][40]

YopE functions as a GTPase-activating protein for members of the Rho family of GTPases such as RAC1. YopT is a cysteine protease that inhibits RhoA by removing the isoprenyl group, which is important for localizing the protein to the cell membrane. YopE and YopT has been proposed to function to limit YopB/D-induced cytolysis. This might limit the function of YopB/D to create the pores used for Yop insertion into host cells and prevent YopB/D-induced rupture of host cells and release of cell contents that would attract and stimulate immune system responses.[42]

YopJ is an acetyltransferase that binds to a conserved α-helix of MAPK kinases.[43] YopJ acetylates MAPK kinases at serines and threonines that are normally phosphorylated during activation of the MAP kinase cascade.[44][45] YopJ is activated in eukaryotic cells by interaction with target cell phytic acid (IP6).[46] This disruption of host cell protein kinase activity causes apoptosis of macrophages, and this is proposed to be important for the establishment of infection and for evasion of the host immune response. YopO is a protein kinase also known as Yersinia protein kinase A (YpkA). YopO is a potent inducer of human macrophage apoptosis.[47]

It has also been suggested that a bacteriophage – Ypφ – may have been responsible for increasing the virulence of this organism.[48]Depending on which form of the plague infects the individual, the plague develops a different illness; however, the plague overall affects the host cell's ability to communicate with the immune system, hindering the body bringing phagocytic cells to the area of infection.[49][50]

Other animals

Y. pestis is a versatile killer. In addition to rodents and humans, it is known to have killed camels, chickens, and pigs.[51] Domestic dogs and cats are susceptible to plague, as well, but cats are more likely to develop illness when infected. In either, the symptoms are similar to those experienced by humans, and can be deadly to the animal. People can be exposed by coming into contact with an infected animal, or inhaling infectious droplets that a sick dog or cat has coughed into the air.[52][53]

Immunity

Main article: Plague vaccine

A formalin-inactivated vaccine was available in the United States for adults in 1993[54] at high risk of contracting the plague until removal from the market by the Food and Drug Administration. It was of limited effectiveness and could cause severe inflammation. Experiments with genetic engineering of a vaccine based on F1 and V antigens are underway and show promise. However, bacteria lacking antigen F1 are still virulent, and the V antigens are sufficiently variable such that vaccines composed of these antigens may not be fully protective.[55] The United States Army Medical Research Institute of Infectious Diseases has found that an experimental F1/V antigen-based vaccine protects crab-eating macaques, but fails to protect African green monkey species.[56] A systematic review by the Cochrane Collaboration found no studies of sufficient quality to make any statement on the efficacy of the vaccine.[57]

History

Alexandre Yersin
Y. pestis isolated by Ricardo Jorge during the 1899 Porto plague outbreak

In 1894, two bacteriologists, Alexandre Yersin of Switzerland and Kitasato Shibasaburō of Japan, independently isolated in Hong Kong the bacterium responsible for the 1894 Hong Kong plague. Though both investigators reported their findings, a series of contradictory statements by Kitasato eventually led to the acceptance of Yersin as the primary discoverer of the organism. Yersin named it Pasteurella pestis in honor of the Pasteur Institute, where he worked. In 1967, it was moved to a new genus and renamed Yersinia pestis in his honor. Yersin also noted that rats were affected by plague not only during plague epidemics, but also often preceding such epidemics in humans and that plague was regarded by many locals as a disease of rats.[58][59][4]

In 1898, French scientist Paul-Louis Simond (who had also come to China to battle the Third Pandemic) discovered the rat–flea vector that drives the disease. He had noted that persons who became ill did not have to be in close contact with each other to acquire the disease. In Yunnan, China, inhabitants would flee from their homes as soon as they saw dead rats, and on the island of Formosa (Taiwan), residents considered the handling of dead rats heightened the risks of developing plague. These observations led him to suspect that the flea might be an intermediary factor in the transmission of plague, since people acquired plague only if they were in contact with rats that had died less than 24 hours before. In a now classic experiment, Simond demonstrated how a healthy rat died of the plague after infected fleas had jumped to it from a rat that had recently died of the plague.[60] The outbreak spread to Chinatown, San Francisco, from 1900 to 1904 and then to Oakland and the East Bay from 1907 to 1909.[61] It has been present in the rodents of western North America ever since, as fear of the consequences of the outbreak on trade caused authorities to hide the dead of the Chinatown residents long enough for the disease to be passed to widespread species of native rodents in outlying areas.[62]

Three main strains are recognised: Y. p. antiqua, which caused a plague pandemic in the sixth century; Y. p. medievalis, which caused the Black Death and subsequent epidemics during the second pandemic wave; and Y. p. orientalis, which is responsible for current plague outbreaks.[63]

Outbreaks

Black death- Molecula/immunological detection of plague agent Yersinia pestis in ancient burial sites in Europe in bones star and dental pulp without star[64]
Further information: Black death, Bubonic plague, and List of epidemics

Yersinia pestis has been discovered in archaeological finds from the Late Bronze Age (~3800 BP),[65] and is also well known for the Black Death many centuries later.

  • In 2008, plague was commonly found in sub-Saharan Africa and Madagascar, areas that accounted for over 95% of the reported cases.[7]
  • On November 3, 2019, two cases of pneumonic plague were diagnosed at a hospital in Beijing's Chaoyang district, prompting fears of an outbreak. The patient was a middle-aged man with fever, who had complained of difficulty breathing for some ten days, accompanied by his wife with similar symptoms.[66] Police quarantined the emergency room at the hospital and controls were placed on Chinese news aggregators.[66] On 18 November a third case was reported, in a 55-year-old man from Xilingol League, one of the twelve Mongolian autonomous regions in Northern China. The patient received treatment, and 28 symptomless contacts were placed in quarantine.[67]
  • In July 2020, officials increased precautions after a case of bubonic plague was confirmed in Bayannur, a city in China's Inner Mongolia autonomous region. The patient was quarantined and treated. According to China's Global Times, a second suspected case was also investigated, and a level 3 alert was issued, in effect until the end of the year. It forbade hunting and eating of animals that could carry plague, and called on the public to report suspected cases.[68]

Research

  • In September 2009, the death of Malcolm Casadaban, a molecular genetics professor at the University of Chicago, was linked to his work on a weakened laboratory strain of Y. pestis.[69] Hemochromatosis was hypothesised to be a predisposing factor in Casadaban's death from this attenuated strain used for research.[70]
  • In 2010, researchers in Germany definitively established, using PCR evidence from samples obtained from Black Death victims, that Y. pestis was the cause of the medieval Black Death.[71]
  • In 2011, the first genome of Y. pestis isolated from Black Death victims was published, and concluded that this medieval strain was ancestral to most modern forms of Y. pestis.[72]
  • In 2015, Cell published results from a study of ancient graves.[73] Plasmids of Y. pestis were detected in archaeological samples of the teeth of seven Bronze Age individuals, in the Afanasievo culture in Siberia, the Corded Ware culture in Estonia, the Sintashta culture in Russia, the Unetice culture in Poland, and the Andronovo culture in Siberia.[74] In 2018, the emergence and spread of the pathogen during the Neolithic decline (as far back as 6,000 years ago) was published.[75] A site in Sweden was the source of the DNA evidence and trade networks were proposed as the likely avenue of spread rather than migrations of populations. There is evidence that suggests Y. pestis may have originated in Europe in the Cucuteni–Trypillia culture, not in Asia as is more commonly believed.[75]
University of Oslo researchers determined that while Yersinia pestis was spread over the Silk road their paper indicates it may have been gerbils (not rats) that where the cause[76]
  • DNA evidence published in 2015 indicates Y. pestis infected humans 5,000 years ago in Bronze Age Eurasia,[73] but genetic changes that made it highly virulent did not occur until about 4,000 years ago.[77] The highly virulent version capable of transmission by fleas through rodents, humans, and other mammals was found in two individuals associated with the Srubnaya culture from the Samara region in Russia from around 3,800 years ago and an Iron Age individual from Kapan, Armenia from around 2,900 years ago.[77][73] This indicates that at least two lineages of Y. pestis were circulating during the Bronze Age in Eurasia.[77] The Y. pestis bacterium has a relatively large number of nonfunctioning genes and three "ungainly" plasmids, suggesting an origin less than 20,000 years ago.[51]
  • On September 8, 2016, the Y. pestis bacterium was identified from DNA in teeth found at a Crossrail building site in London. The human remains were found to be victims of the Great Plague of London, which lasted from 1665 to 1666.[78]
  • On January 15, 2018, researchers at the University of Oslo and the University of Ferrara suggested that humans and their parasites (most likely fleas and lice at the time) were the biggest carriers of the plague.[79][80]
  • In 2021, researchers found a 5,000-year-old victim of Y. pestis, the world's oldest-known, in hunter-gatherer remains in the modern Latvian and Estonian border area.[81]

References

  1. ↑ Ryan, KJ; Ray, CG, eds. (2004). Sherris Medical Microbiology (4th ed.). McGraw Hill. pp. 484–88. ISBN 978-0-8385-8529-0.
  2. ↑ Yersin, Alexandre (1894). "La peste bubonique à Hong-Kong". Annales de l'Institut Pasteur (in français). 8: 662–67. Archived from the original on 2021-07-11. Retrieved 2022-10-29.
  3. ↑ Bockemühl, J (April 1994). "100 years after the discovery of the plague-causing agent—importance and veneration of Alexandre Yersin in Vietnam today". Immunitat und Infektion. 22 (2): 72–5. PMID 7959865.
  4. 1 2 Howard-Jones, N. (1973). "Was Shibasaburo Kitasato the co-discoverer of the plague bacillus?". Perspectives in Biology and Medicine. 16 (2): 292–307. doi:10.1353/pbm.1973.0034. ISSN 0031-5982. Archived from the original on 30 October 2022. Retrieved 1 November 2022.
  5. ↑ "Taxonomy browser (Yersinia pestis)". www.ncbi.nlm.nih.gov. Archived from the original on 11 November 2022. Retrieved 5 November 2022.
  6. ↑ "Plague FAQ". CDC. 15 November 2021. Archived from the original on 14 March 2017. Retrieved 29 October 2022.
  7. 1 2 "The Plague" Archived 2022-10-17 at the Wayback Machine, Centers for Disease Control and Prevention, Oct. 2017 Public Domain This article incorporates text from this source, which is in the public domain.
  8. ↑ Stenseth, Nils Chr.; Tao, Yuxin; Zhang, Chutian; Bramanti, Barbara; Büntgen, Ulf; Cong, Xianbin; Cui, Yujun; Zhou, Hu; Dawson, Lorna A.; Mooney, Sacha J.; Li, Dong; Fell, Henry G.; Cohn, Samuel; Sebbane, Florent; Slavin, Philip; Liang, Wannian; Tong, Howell; Yang, Ruifu; Xu, Lei (20 December 2022). "No evidence for persistent natural plague reservoirs in historical and modern Europe". Proceedings of the National Academy of Sciences. 119 (51): e2209816119. doi:10.1073/pnas.2209816119. Archived from the original on 23 January 2023. Retrieved 5 February 2023.
  9. 1 2 Collins FM (1996). Baron S; et al. (eds.). Pasteurella, Yersinia, and Francisella. In: Baron's Medical Microbiology (4th ed.). Univ. of Texas Medical Branch. ISBN 978-0-9631172-1-2. Archived from the original on 2021-03-09. Retrieved 2022-10-29.
  10. ↑ Stackebrandt, Erko; Dworkin, Martin; Falkow, Stanley; Rosenberg, Eugene; Karl-Heinz Schleifer (2005). The Prokaryotes: A Handbook on the Biology of Bacteria: Volume 6: Proteobacteria: Gamma Subclass. Berlin: Springer. ISBN 978-0-387-25499-9.
  11. ↑ "Yersinia pestis". pubchem.ncbi.nlm.nih.gov. Archived from the original on 30 October 2022. Retrieved 30 October 2022.
  12. ↑ Deng W, Burland V, Plunkett G, Boutin A, Mayhew GF, Liss P, et al. (August 2002). "Genome sequence of Yersinia pestis KIM". Journal of Bacteriology. 184 (16): 4601–11. doi:10.1128/JB.184.16.4601-4611.2002. PMC 135232. PMID 12142430.
  13. 1 2 3 Parkhill J, Wren BW, Thomson NR, Titball RW, Holden MT, Prentice MB, et al. (October 2001). "Genome sequence of Yersinia pestis, the causative agent of plague". Nature. 413 (6855): 523–7. Bibcode:2001Natur.413..523P. doi:10.1038/35097083. PMID 11586360.
  14. ↑ Chain PS, Hu P, Malfatti SA, Radnedge L, Larimer F, Vergez LM, et al. (June 2006). "Complete genome sequence of Yersinia pestis strains Antiqua and Nepal516: evidence of gene reduction in an emerging pathogen". Journal of Bacteriology. 188 (12): 4453–63. doi:10.1128/JB.00124-06. PMC 1482938. PMID 16740952.
  15. 1 2 Song, Yajun; Tong, Zongzhong; Wang, Jin; Wang, Li; Guo, Zhaobiao; Han, Yanpin; Zhang, Jianguo; Pei, Decui; Zhou, Dongsheng; Qin, Haiou; Pang, Xin (2004-06-30). "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans". DNA Research. 11 (3): 179–197. doi:10.1093/dnares/11.3.179. ISSN 1340-2838. PMID 15368893. Archived from the original on 2022-10-19. Retrieved 2022-10-29.
  16. 1 2 Hinnebusch BJ, Rudolph AE, Cherepanov P, Dixon JE, Schwan TG, Forsberg A (April 2002). "Role of Yersinia murine toxin in survival of Yersinia pestis in the midgut of the flea vector". Science. 296 (5568): 733–5. Bibcode:2002Sci...296..733H. doi:10.1126/science.1069972. PMID 11976454. S2CID 34770234.
  17. 1 2 Lathem WW, Price PA, Miller VL, Goldman WE (January 2007). "A plasminogen-activating protease specifically controls the development of primary pneumonic plague". Science. 315 (5811): 509–13. Bibcode:2007Sci...315..509L. doi:10.1126/science.1137195. PMID 17255510. S2CID 39881239.
  18. ↑ Han, Yanping; Fang, Haihong; Liu, Lei; Zhou, Dongsheng (2016). "Genetic Regulation of Yersinia pestis". Advances in Experimental Medicine and Biology. 918: 223–256. doi:10.1007/978-94-024-0890-4_8. ISSN 0065-2598. Archived from the original on 30 October 2022. Retrieved 30 October 2022.
  19. ↑ Hu, Ping; Elliott, Jeffrey; McCready, Paula; Skowronski, Evan; Garnes, Jeffrey; Kobayashi, Arthur; Brubaker, Robert R.; Garcia, Emilio (October 1998). "Structural Organization of Virulence-Associated Plasmids of Yersinia pestis". Journal of Bacteriology. 180 (19): 5192–5202. ISSN 0021-9193. Archived from the original on 11 November 2022. Retrieved 7 November 2022.
  20. ↑ Hixson KK, Adkins JN, Baker SE, Moore RJ, Chromy BA, Smith RD, et al. (November 2006). "Biomarker candidate identification in Yersinia pestis using organism-wide semiquantitative proteomics". Journal of Proteome Research. 5 (11): 3008–17. doi:10.1021/pr060179y. PMID 17081052.
  21. ↑ Schiano CA, Koo JT, Schipma MJ, Caulfield AJ, Jafari N, Lathem WW (May 2014). "Genome-wide analysis of small RNAs expressed by Yersinia pestis identifies a regulator of the Yop-Ysc type III secretion system". Journal of Bacteriology. 196 (9): 1659–70. doi:10.1128/JB.01456-13. PMC 3993326. PMID 24532772.
  22. ↑ Cornelis GR, Boland A, Boyd AP, Geuijen C, Iriarte M, Neyt C, et al. (December 1998). "The virulence plasmid of Yersinia, an antihost genome". Microbiology and Molecular Biology Reviews. 62 (4): 1315–52. doi:10.1128/MMBR.62.4.1315-1352.1998. PMC 98948. PMID 9841674.
  23. ↑ Yan Y, Su S, Meng X, Ji X, Qu Y, Liu Z, et al. (2013). "Determination of sRNA expressions by RNA-seq in Yersinia pestis grown in vitro and during infection". PLOS ONE. 8 (9): e74495. Bibcode:2013PLoSO...874495Y. doi:10.1371/journal.pone.0074495. PMC 3770706. PMID 24040259.
  24. ↑ Barbieri, R.; Signoli, M.; Chevé, D.; Costedoat, C.; Tzortzis, S.; Aboudharam, G.; Raoult, D.; Drancourt, M. (January 2021). "Yersinia pestis: the Natural History of Plague". Clinical Microbiology Reviews. 34 (1). doi:10.1128/CMR.00044-19. Archived from the original on 9 September 2022. Retrieved 2 November 2022.
  25. ↑ "CDC Plague | Frequently Asked Questions (FAQ) About Plague". emergency.cdc.gov. 21 February 2019. Archived from the original on 28 August 2008. Retrieved 6 November 2022.
  26. ↑ Bhunia, Arun (22 November 2007). Foodborne Microbial Pathogens: Mechanisms and Pathogenesis. Springer Science & Business Media. p. 236. ISBN 978-0-387-74537-4. Archived from the original on 11 November 2022. Retrieved 11 November 2022.
  27. ↑ Meyer KF (August 1957). "The natural history of plague and psittacosis". Public Health Reports. 72 (8): 705–19. doi:10.2307/4589874. JSTOR 4589874. PMC 2031327. PMID 13453634.
  28. ↑ von Reyn CF, Weber NS, Tempest B, Barnes AM, Poland JD, Boyce JM, Zalma V (October 1977). "Epidemiologic and clinical features of an outbreak of bubonic plague in New Mexico". The Journal of Infectious Diseases. 136 (4): 489–94. doi:10.1093/infdis/136.4.489. PMID 908848.
  29. ↑ Pauli JN, Buskirk SW, Williams ES, Edwards WH (January 2006). "A plague epizootic in the black-tailed prairie dog (Cynomys ludovicianus)". Journal of Wildlife Diseases. 42 (1): 74–80. doi:10.7589/0090-3558-42.1.74. PMID 16699150. S2CID 9716200.
  30. ↑ Webb CT, Brooks CP, Gage KL, Antolin MF (April 2006). "Classic flea-borne transmission does not drive plague epizootics in prairie dogs". Proceedings of the National Academy of Sciences of the United States of America. 103 (16): 6236–41. Bibcode:2006PNAS..103.6236W. doi:10.1073/pnas.0510090103. PMC 1434514. PMID 16603630.
  31. ↑ Bertherat E, Bekhoucha S, Chougrani S, Razik F, Duchemin JB, Houti L, et al. (October 2007). "Plague reappearance in Algeria after 50 years, 2003". Emerging Infectious Diseases. 13 (10): 1459–62. doi:10.3201/eid1310.070284. PMC 2851531. PMID 18257987.
  32. ↑ Zhou D, Han Y, Yang R (January 2006). "Molecular and physiological insights into plague transmission, virulence and etiology". Microbes and Infection. 8 (1): 273–84. doi:10.1016/j.micinf.2005.06.006. PMID 16182593.
  33. ↑ Hinnebusch BJ, Perry RD, Schwan TG (July 1996). "Role of the Yersinia pestis hemin storage (hms) locus in the transmission of plague by fleas". Science. 273 (5273): 367–70. Bibcode:1996Sci...273..367H. doi:10.1126/science.273.5273.367. PMID 8662526. S2CID 37512575.
  34. ↑ Erickson DL, Waterfield NR, Vadyvaloo V, Long D, Fischer ER, Ffrench-Constant R, Hinnebusch BJ (November 2007). "Acute oral toxicity of Yersinia pseudotuberculosis to fleas: implications for the evolution of vector-borne transmission of plague". Cellular Microbiology. 9 (11): 2658–66. doi:10.1111/j.1462-5822.2007.00986.x. PMID 17587333. S2CID 36769530.
  35. ↑ Jarrett CO, Deak E, Isherwood KE, Oyston PC, Fischer ER, Whitney AR, et al. (August 2004). "Transmission of Yersinia pestis from an infectious biofilm in the flea vector". The Journal of Infectious Diseases. 190 (4): 783–92. doi:10.1086/422695. PMID 15272407.
  36. ↑ Erickson DL, Jarrett CO, Wren BW, Hinnebusch BJ (February 2006). "Serotype differences and lack of biofilm formation characterize Yersinia pseudotuberculosis infection of the Xenopsylla cheopis flea vector of Yersinia pestis". Journal of Bacteriology. 188 (3): 1113–9. doi:10.1128/jb.188.3.1113-1119.2006. PMC 1347331. PMID 16428415.
  37. 1 2 Hinnebusch BJ, Erickson DL (2008). "Yersinia pestis biofilm in the flea vector and its role in the transmission of plague". Current Topics in Microbiology and Immunology. 322: 229–48. doi:10.1007/978-3-540-75418-3_11. ISBN 978-3-540-75417-6. PMC 3727414. PMID 18453279.
  38. ↑ Salyers AA, Whitt DD (2002). Bacterial Pathogenesis: A Molecular Approach (2nd ed.). ASM Press. pp. 207–212.
  39. ↑ Viboud GI, Bliska JB (2005). "Yersinia outer proteins: role in modulation of host cell signaling responses and pathogenesis". Annual Review of Microbiology. 59 (#1): 69–89. doi:10.1146/annurev.micro.59.030804.121320. PMID 15847602.
  40. 1 2 de la Puerta, María Luisa; Trinidad, Antonio G.; Rodríguez, María del Carmen; Bogetz, Jori; Sánchez Crespo, Mariano; Mustelin, Tomas; Alonso, Andrés; Bayón, Yolanda (16 February 2009). "Characterization of New Substrates Targeted By Yersinia Tyrosine Phosphatase YopH". PLoS ONE. 4 (2): e4431. doi:10.1371/journal.pone.0004431. ISSN 1932-6203. Archived from the original on 11 November 2022. Retrieved 9 November 2022.
  41. ↑ Aktories, Klaus; Just, Ingo (2 May 2007). Special Issue on Emerging Bacterial Toxins. Springer Science & Business Media. p. 73. ISBN 978-3-540-27115-4.
  42. ↑ Mejía E, Bliska JB, Viboud GI (January 2008). "Yersinia controls type III effector delivery into host cells by modulating Rho activity". PLOS Pathogens. 4 (1): e3. doi:10.1371/journal.ppat.0040003. PMC 2186360. PMID 18193942.
  43. ↑ Hao YH, Wang Y, Burdette D, Mukherjee S, Keitany G, Goldsmith E, Orth K (January 2008). Kobe B (ed.). "Structural requirements for Yersinia YopJ inhibition of MAP kinase pathways". PLOS ONE. 3 (1): e1375. Bibcode:2008PLoSO...3.1375H. doi:10.1371/journal.pone.0001375. PMC 2147050. PMID 18167536.
  44. ↑ Mukherjee S, Keitany G, Li Y, Wang Y, Ball HL, Goldsmith EJ, Orth K (May 2006). "Yersinia YopJ acetylates and inhibits kinase activation by blocking phosphorylation". Science. 312 (5777): 1211–4. Bibcode:2006Sci...312.1211M. doi:10.1126/science.1126867. PMID 16728640. S2CID 13101320.
  45. ↑ Mittal R, Peak-Chew SY, McMahon HT (December 2006). "Acetylation of MEK2 and I kappa B kinase (IKK) activation loop residues by YopJ inhibits signaling". Proceedings of the National Academy of Sciences of the United States of America. 103 (49): 18574–9. Bibcode:2006PNAS..10318574M. doi:10.1073/pnas.0608995103. PMC 1654131. PMID 17116858.
  46. ↑ Mittal R, Peak-Chew SY, Sade RS, Vallis Y, McMahon HT (June 2010). "The acetyltransferase activity of the bacterial toxin YopJ of Yersinia is activated by eukaryotic host cell inositol hexakisphosphate". The Journal of Biological Chemistry. 285 (26): 19927–34. doi:10.1074/jbc.M110.126581. PMC 2888404. PMID 20430892.
  47. ↑ Park H, Teja K, O'Shea JJ, Siegel RM (May 2007). "The Yersinia effector protein YpkA induces apoptosis independently of actin depolymerization". Journal of Immunology. 178 (10): 6426–34. doi:10.4049/jimmunol.178.10.6426. PMID 17475872.
  48. ↑ Derbise A, Chenal-Francisque V, Pouillot F, Fayolle C, Prévost MC, Médigue C, et al. (February 2007). "A horizontally acquired filamentous phage contributes to the pathogenicity of the plague bacillus". Molecular Microbiology. 63 (4): 1145–57. doi:10.1111/j.1365-2958.2006.05570.x. PMID 17238929. S2CID 30862265.
  49. ↑ Marketon, Melanie M.; DePaolo, R. William; DeBord, Kristin L.; Jabri, Bana; Schneewind, Olaf (9 September 2005). "Plague Bacteria Target Immune Cells During Infection". Science (New York, N.Y.). 309 (5741): 1739–1741. doi:10.1126/science.1114580. ISSN 0036-8075. Archived from the original on 11 November 2022. Retrieved 5 November 2022.
  50. ↑ Li, Bei; Yang, Ruifu (2008). "Interaction between Yersinia pestis and the Host Immune System". Infection and Immunity. 76 (5): 1804–1811. doi:10.1128/IAI.01517-07. ISSN 0019-9567. Archived from the original on 11 November 2022. Retrieved 6 November 2022.
  51. 1 2 Kelly J (2005). Great mortality : an intimate history of the Black Death (1st ed.). London [u.a.]: Fourth Estate. p. 35. ISBN 978-0007150694.
  52. ↑ "Cats – Healthy Pets Healthy People". Centers for Disease Control and Prevention. 2016-05-13. Archived from the original on 2014-05-12. Retrieved 2016-11-25.
  53. ↑ "Dogs – Healthy Pets Healthy People". Centers for Disease Control and Prevention. 2020-02-21. Archived from the original on 2020-06-29. Retrieved 2020-06-30.
  54. ↑ Sheet, The Pink (21 Nov 1994). "GREER LABORATORIES BUBONIC PLAGUE VACCINE APPROVED". Pink Sheet.
  55. ↑ Welkos S, Pitt ML, Martinez M, Friedlander A, Vogel P, Tammariello R (May 2002). "Determination of the virulence of the pigmentation-deficient and pigmentation-/plasminogen activator-deficient strains of Yersinia pestis in non-human primate and mouse models of pneumonic plague". Vaccine. 20 (17–18): 2206–14. doi:10.1016/S0264-410X(02)00119-6. PMID 12009274.
  56. ↑ Pitt ML (13 October 2004). Non-human primates as a model for pneumonic plague (PDF). Animals Models and Correlates of Protection for Plague Vaccines Workshop, Gaithersburg, Maryland. Center for Biologics Evaluation and Research (Food and Drug Administration, Department of Health and Human Resources). pp. 222–248. Archived from the original (PDF) on 25 December 2004.
  57. ↑ Jefferson T, Demicheli V, Pratt M (2000). Jefferson T (ed.). "Vaccines for preventing plague". The Cochrane Database of Systematic Reviews (2): CD000976. doi:10.1002/14651858.CD000976. PMC 6532692. PMID 10796565. Art. No. CD000976.
  58. ↑ Hawgood, Barbara J. (August 2008). "Alexandre Yersin (1863-1943): discoverer of the plague bacillus, explorer and agronomist". Journal of Medical Biography. 16 (3): 167–172. doi:10.1258/jmb.2007.007017. ISSN 0967-7720. Archived from the original on 18 May 2022. Retrieved 31 October 2022.
  59. ↑ Solomon, T. (June 1995). "Alexandre Yersin and the plague bacillus". The Journal of Tropical Medicine and Hygiene. 98 (3): 209–212. ISSN 0022-5304. Archived from the original on 1 March 2022. Retrieved 31 October 2022.
  60. ↑ "The Plague". Association Amicale Sante Navale et d'Outremer. Archived from the original on 4 September 2012.
  61. ↑ "On This Day: San Francisco Bubonic Plague Outbreak Begins". Finding Dulcinea. Archived from the original on 2017-12-01. Retrieved 2017-11-25.
  62. ↑ Chase, M. (2004). The Barbary Plague: The Black Death in Victorian San Francisco. Random House Trade Paperbacks.
  63. ↑ Achtman M, Zurth K, Morelli G, Torrea G, Guiyoule A, Carniel E (November 1999). "Yersinia pestis, the cause of plague, is a recently emerged clone of Yersinia pseudotuberculosis". Proceedings of the National Academy of Sciences of the United States of America. 96 (24): 14043–8. Bibcode:1999PNAS...9614043A. doi:10.1073/pnas.96.24.14043. PMC 24187. PMID 10570195.
  64. ↑ Malou, Nada; Tran, Thi-Nguyen-Ny; Nappez, Claude; Signoli, Michel; Le Forestier, Cyrille; Castex, Dominique; Drancourt, Michel; Raoult, Didier (9 February 2012). "Immuno-PCR - A New Tool for Paleomicrobiology: The Plague Paradigm". PLoS ONE. 7 (2): e31744. doi:10.1371/journal.pone.0031744. ISSN 1932-6203. Retrieved 11 November 2022.
  65. ↑ Spyrou MA, Tukhbatova RI, Wang CC, Valtueña AA, Lankapalli AK, Kondrashin VV, Tsybin VA, Khokhlov A, Kühnert D, Herbig A, Bos KI, Krause J (June 2018). "Analysis of 3800-year-old Yersinia pestis genomes suggests Bronze Age origin for bubonic plague". Nature Communications. 9 (1): 2234. Bibcode:2018NatCo...9.2234S. doi:10.1038/s41467-018-04550-9. PMC 5993720. PMID 29884871.
  66. 1 2 Wee SL (2019-11-13). "Pneumonic Plague Is Diagnosed in China". The New York Times. ISSN 0362-4331. Archived from the original on 2019-11-13. Retrieved 2019-11-13.
  67. ↑ "Bubonic plague: Third case reported in China". 19 November 2019. Archived from the original on 19 December 2019. Retrieved 29 October 2022.
  68. ↑ "China bubonic plague: Inner Mongolia takes precautions after case". BBC. 2020-07-06. Archived from the original on 2020-07-06. Retrieved 2020-07-06.
  69. ↑ Sadovi, Carlos (2009-09-19). "U. of C. researcher dies after exposure to plague bacteria". Chicago Breaking News Center. Archived from the original on 2010-04-07. Retrieved 2010-03-03.
  70. ↑ Randall T (Feb 25, 2011). "Plague Death Came Within Hours, Spurred by Scientist's Medical Condition". Archived from the original on 2015-01-09. Retrieved 2022-10-29.
  71. ↑ Haensch S, Bianucci R, Signoli M, Rajerison M, Schultz M, Kacki S, et al. (October 2010). "Distinct clones of Yersinia pestis caused the black death". PLOS Pathogens. 6 (10): e1001134. doi:10.1371/journal.ppat.1001134. PMC 2951374. PMID 20949072.
  72. ↑ Bos KI, Schuenemann VJ, Golding GB, Burbano HA, Waglechner N, Coombes BK, et al. (October 2011). "A draft genome of Yersinia pestis from victims of the Black Death". Nature. 478 (7370): 506–10. Bibcode:2011Natur.478..506B. doi:10.1038/nature10549. PMC 3690193. PMID 21993626.
  73. 1 2 3 Rasmussen S, Allentoft ME, Nielsen K, Orlando L, Sikora M, Sjögren KG, et al. (October 2015). "Early divergent strains of Yersinia pestis in Eurasia 5,000 years ago". Cell. 163 (3): 571–82. doi:10.1016/j.cell.2015.10.009. PMC 4644222. PMID 26496604. This article contains quotations from this source, which is available under the Creative Commons Attribution 4.0 International (CC BY 4.0) Archived 2017-10-16 at the Wayback Machine license.
  74. ↑ Zimmer C (2015-10-22). "In Ancient DNA, Evidence of Plague Much Earlier Than Previously Known". The New York Times. ISSN 0362-4331. Archived from the original on 2020-04-17. Retrieved 2020-04-16.
  75. 1 2 Rascovan N, Sjögren KG, Kristiansen K, Nielsen R, Willerslev E, Desnues C, Rasmussen S (January 2019). "Emergence and Spread of Basal Lineages of Yersinia pestis during the Neolithic Decline". Cell. 176 (1–2): 295–305.e10. doi:10.1016/j.cell.2018.11.005. PMID 30528431.
  76. ↑ Schmid, Boris V.; Büntgen, Ulf; Easterday, W. Ryan; Ginzler, Christian; Walløe, Lars; Bramanti, Barbara; Stenseth, Nils Chr. (10 March 2015). "Climate-driven introduction of the Black Death and successive plague reintroductions into Europe". Proceedings of the National Academy of Sciences. 112 (10): 3020–3025. doi:10.1073/pnas.1412887112. ISSN 0027-8424. Retrieved 5 February 2023.
  77. 1 2 3 Spyrou MA, Tukhbatova RI, Wang CC, Valtueña AA, Lankapalli AK, Kondrashin VV, et al. (June 2018). "Analysis of 3800-year-old Yersinia pestis genomes suggests Bronze Age origin for bubonic plague". Nature Communications. 9 (1): 2234. Bibcode:2018NatCo...9.2234S. doi:10.1038/s41467-018-04550-9. PMC 5993720. PMID 29884871. This article contains quotations from this source, which is available under the Creative Commons Attribution 4.0 International (CC BY 4.0) Archived 2017-10-16 at the Wayback Machine license.
  78. ↑ "DNA of bacteria responsible for London Great Plague of 1665 identified for first time". Archived from the original on 2021-12-20. Retrieved 2022-10-29.
  79. ↑ "Don't blame the rats: Human fleas and lice likely spread Black Death". CBC News. Archived from the original on 2021-03-23. Retrieved 2022-10-29.
  80. ↑ Dean KR, Krauer F, Walløe L, Lingjærde OC, Bramanti B, Stenseth NC, Schmid BV (February 2018). "Human ectoparasites and the spread of plague in Europe during the Second Pandemic". Proceedings of the National Academy of Sciences of the United States of America. 115 (6): 1304–1309. doi:10.1073/pnas.1715640115. PMC 5819418. PMID 29339508.
  81. ↑ Susat J (4 September 2021). "A 5,000-year-old hunter-gatherer already plagued by Yersinia pestis". Cell. 35 (13): 506–10. doi:10.1016/j.celrep.2021.109278. PMID 34192537. S2CID 235697166.
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