EIF1

Eukaryotic translation initiation factor 1 (eIF1) is a protein that in humans is encoded by the EIF1 gene. It is related to yeast SUI1.[3][4][5]

EIF1
Available structures
PDBHuman UniProt search: PDBe RCSB
Identifiers
AliasesEIF1, A121, EIF-1, ISO1, SUI1, eukaryotic translation initiation factor 1, Eukaryotic initiation factor 1
External IDsHomoloGene: 130538 GeneCards: EIF1
Orthologs
SpeciesHumanMouse
Entrez

10209

n/a

Ensembl

ENSG00000173812

n/a

UniProt

P41567

n/a

RefSeq (mRNA)

NM_005801

n/a

RefSeq (protein)

NP_005792

n/a

Location (UCSC)Chr 17: 41.69 – 41.69 Mbn/a
PubMed search[2]n/a
Wikidata
View/Edit Human

eIF1 interacts with the eukaryotic small (40S) ribosomal subunit and eIF3, and is a component of the 43S preinitiation complex (PIC).[6] eIF1 and eIF1A bind cooperatively to the 40S to stabilize an "open" conformation of the preinitiation complex (PIC) during eukaryotic translation initiation.[6] eIF1 binds to a region near the ribosomal P-site in the 40S subunit and functions in a manner similar to the structurally related bacterial counterpart IF3.[7]

Structure

eIF1 is a conserved translation protein in all eukaryotic cells that is responsible for the investigation of codon-anticodon mismatches during the initiation of translation. In order to determine the structure of human eIF1, an experiment with N-terminal His tag and eIF1 are conducted via using NMR spectroscopy. Scientists have discovered a binding site by generating yeast mutation and study the neighbor conserved residues located in the same region. GST pull-down experiments has shown that eIF1 binds precisely to the p110 subunit of eIF3 as a result explaining eIF1 recruiting. [8]

Function

The function of eIf1 has some hidden aspects. However, in all eukaryotic cells initiation of mRNA translation starts with scanning via ribosomal 43S preinitiation complexes starting from the 5’ end of the mRNA. Next, induction via eIF1 and eIF1A are needed to disclose the conformation of the 40S subunit in order to induce DEAD-box RNA helicase eIF4A, its cofactor eIF4B, and eIF4G activity.[9]   

See also

References
  1. GRCh38: Ensembl release 89: ENSG00000173812 - Ensembl, May 2017
  2. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  3. Fields C, Adams MD (January 1994). "Expressed sequence tags identify a human isolog of the suil translation initiation factor". Biochemical and Biophysical Research Communications. 198 (1): 288–91. doi:10.1006/bbrc.1994.1040. PMID 7904817.
  4. Sheikh MS, Fernandez-Salas E, Yu M, Hussain A, Dinman JD, Peltz SW, Huang Y, Fornace AJ (June 1999). "Cloning and characterization of a human genotoxic and endoplasmic reticulum stress-inducible cDNA that encodes translation initiation factor 1(eIF1(A121/SUI1))". The Journal of Biological Chemistry. 274 (23): 16487–93. doi:10.1074/jbc.274.23.16487. PMID 10347211.
  5. "Entrez Gene: EIF1 eukaryotic translation initiation factor 1".
  6. Aitken CE, Lorsch JR (June 2012). "A mechanistic overview of translation initiation in eukaryotes". Nature Structural & Molecular Biology. 19 (6): 568–76. doi:10.1038/nsmb.2303. PMID 22664984. S2CID 9201095.
  7. Fraser CS (July 2015). "Quantitative studies of mRNA recruitment to the eukaryotic ribosome". Biochimie. 114: 58–71. doi:10.1016/j.biochi.2015.02.017. PMC 4458453. PMID 25742741.
  8. Fletcher CM, Pestova TV, Hellen CU, Wagner G (May 1999). "Structure and interactions of the translation initiation factor eIF1". The EMBO Journal. 18 (9): 2631–7. doi:10.1093/emboj/18.9.2631. PMC 1171342. PMID 10228174.
  9. Jackson RJ, Hellen CU, Pestova TV (February 2010). "The mechanism of eukaryotic translation initiation and principles of its regulation". Nature Reviews. Molecular Cell Biology. 11 (2): 113–27. doi:10.1038/nrm2838. PMC 4461372. PMID 20094052.
  10. "EIF1 - Eukaryotic translation initiation factor 1 - Homo sapiens (Human) - EIF1 gene & protein". www.uniprot.org. Retrieved 2018-09-23.

Further reading
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