Glycine

Glycine[1]
skeletal formula of neutral glycine
skeletal formula of zwitterionic glycine
ball-and-stick model of the gas-phase structure
ball-and-stick model of the zwitterionic solid-state structure
space-filling model of the gas-phase structure
space-filling model of the zwitterionic solid-state structure
Names
IUPAC name
Aminoacetic acid[2]
Other names
2-Aminoethanoic acid, Glycocol
Identifiers
CAS Number
3D model (JSmol)
Abbreviations Gly, G
ChEBI
ChEMBL
ChemSpider
DrugBank
ECHA InfoCard 100.000.248
Edit this at Wikidata
EC Number
  • 200-272-2
  • (HCl): 227-841-8
KEGG
PubChem CID
UNII
InChI
  • InChI=1S/C2H5NH2/c3-1-2(4)5/h1,3H2,(H,4,5) checkY
    Key: DHMQDGOQFOQNFH-UHFFFAOYSA-N checkY
  • InChI=1S/C2H5NO2/c3-1-2(4)5/h1,3H2,(H,4,5)
    Key: DHMQDGOQFOQNFH-UHFFFAOYAW
SMILES
  • C(C(=O)O)N
  • Zwitterion: C(C(=O)[O-])[NH3+]
  • (HCl): C(C(=O)O)N.Cl
Properties
Chemical formula
 C2H5NO2
Molar mass 75.067 g·mol−1
Appearance White solid
Density 1.1607 g/cm3[3]
Melting point 233 °C (451 °F; 506 K) (decomposition)
Solubility in water
 24.99 g/100 mL (25 °C)[4]
Solubility soluble in pyridine
sparingly soluble in ethanol
insoluble in ether
Acidity (pKa) 2.34 (carboxyl), 9.6 (amino)[5]
Magnetic susceptibility (χ)
 -40.3·10−6 cm3/mol
Pharmacology
B05CX03 (WHO)
Hazards
Lethal dose or concentration (LD, LC):
2600 mg/kg (mouse, oral)
Supplementary data page
Glycine (data page)
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
checkY verify (what is checkY☒N ?)
Infobox references

Glycine (symbol Gly or G;[6] /ˈɡlaɪsiːn/)[7] is an amino acid that has a single hydrogen atom as its side chain. It is the simplest stable amino acid (carbamic acid is unstable), with the chemical formula NH2‐CH2‐COOH. Glycine is one of the proteinogenic amino acids. It is encoded by all the codons starting with GG (GGU, GGC, GGA, GGG). Glycine is integral to the formation of alpha-helices in secondary protein structure due to its compact form. For the same reason, it is the most abundant amino acid in collagen triple-helices. Glycine is also an inhibitory neurotransmitter – interference with its release within the spinal cord (such as during a Clostridium tetani infection) can cause spastic paralysis due to uninhibited muscle contraction.

It is the only achiral proteinogenic amino acid. It can fit into hydrophilic or hydrophobic environments, due to its minimal side chain of only one hydrogen atom.

History and etymology

Glycine was discovered in 1820 by the French chemist Henri Braconnot when he hydrolyzed gelatin by boiling it with sulfuric acid.[8] He originally called it "sugar of gelatin",[9][10] but the French chemist Jean-Baptiste Boussingault showed that it contained nitrogen.[11] The American scientist Eben Norton Horsford, then a student of the German chemist Justus von Liebig, proposed the name "glycocoll";[12][13] however, the Swedish chemist Berzelius suggested the simpler name "glycine".[14][15] The name comes from the Greek word γλυκύς "sweet tasting"[16] (which is also related to the prefixes glyco- and gluco-, as in glycoprotein and glucose). In 1858, the French chemist Auguste Cahours determined that glycine was an amine of acetic acid.[17]

Production

Although glycine can be isolated from hydrolyzed protein, this is not used for industrial production, as it can be manufactured more conveniently by chemical synthesis.[18] The two main processes are amination of chloroacetic acid with ammonia, giving glycine and ammonium chloride,[19] and the Strecker amino acid synthesis,[20] which is the main synthetic method in the United States and Japan.[21] About 15 thousand tonnes are produced annually in this way.[22]

Glycine is also cogenerated as an impurity in the synthesis of EDTA, arising from reactions of the ammonia coproduct.[23]

Chemical reactions

Its acid–base properties are most important. In aqueous solution, glycine is amphoteric: below pH = 2.4, it converts to the ammonium cation called glycinium. Above about 9.6, it converts to glycinate.

Glycine functions as a bidentate ligand for many metal ions, forming amino acid complexesss. A typical complex is Cu(glycinate)2, i.e. Cu(H2NCH2CO2)2, which exists both in cis and trans isomers.

With acid chlorides, glycine converts to the amidocarboxylic acid, such as hippuric acid[24] and acetylglycine.[25] With nitrous acid, one obtains glycolic acid (van Slyke determination). With methyl iodide, the amine becomes quaternized to give trimethylglycine, a natural product:

H
3N+
CH
2COO−
 + 3 CH3I → (CH
3)
3N+
CH
2COO−
 + 3 HI

Glycine condenses with itself to give peptides, beginning with the formation of glycylglycine:

2 H
3N+
CH
2COO−
 → H
3N+
CH
2CONHCH
2COO−
 + H2O

Pyrolysis of glycine or glycylglycine gives 2,5-diketopiperazine, the cyclic diamide.

It forms esters with alcohols. They are often isolated as their hydrochloride]], e.g., glycine methyl ester hydrochloride. Otherwise the free ester tends to convert to diketopiperazine.

As a bifunctional molecule, glycine reacts with many reagents. These can be classified into N-centered and carboxylate-center reactions.

Metabolism

Biosynthesis

Glycine is not essential to the human diet, as it is biosynthesized in the body from the amino acid serine, which is in turn derived from 3-phosphoglycerate, but the metabolic capacity for glycine biosynthesis does not satisfy the need for collagen synthesis.[26] In most organisms, the enzyme serine hydroxymethyltransferase catalyses this transformation via the cofactor pyridoxal phosphate:[27]

serine + tetrahydrofolate → glycine + N5,N10-methylene tetrahydrofolate + H2O

In the liver of vertebrates, glycine synthesis is catalyzed by glycine synthase (also called glycine cleavage enzyme). This conversion is readily reversible:[27]

CO2 + NH+
4 + N5,N10-methylene tetrahydrofolate + NADH + H+ ⇌ Glycine + tetrahydrofolate + NAD+

In addition to being synthesized from serine, glycine can also be derived from threonine, choline or hydroxyproline via inter-organ metabolism of the liver and kidneys.[28]

Degradation

Glycine is degraded via three pathways. The predominant pathway in animals and plants is the reverse of the glycine synthase pathway mentioned above. In this context, the enzyme system involved is usually called the glycine cleavage system:[27]

Glycine + tetrahydrofolate + NAD+ ⇌ CO2 + NH+
4 + N5,N10-methylene tetrahydrofolate + NADH + H+

In the second pathway, glycine is degraded in two steps. The first step is the reverse of glycine biosynthesis from serine with serine hydroxymethyl transferase. Serine is then converted to pyruvate by serine dehydratase.[27]

In the third pathway of its degradation, glycine is converted to glyoxylate by D-amino acid oxidase. Glyoxylate is then oxidized by hepatic lactate dehydrogenase to oxalate in an NAD+-dependent reaction.[27]

The half-life of glycine and its elimination from the body varies significantly based on dose.[29] In one study, the half-life varied between 0.5 and 4.0 hours.[29]

Glycine is extremely sensitive to antibiotics which target folate, and blood glycine levels drop severely within a minute of antibiotic injections. Some antibiotics can deplete more than 90% of glycine within a few minutes of being administered.[30]

Physiological function

The principal function of glycine is it act as a precursor to proteins. Most proteins incorporate only small quantities of glycine, a notable exception being collagen, which contains about 35% glycine due to its periodically repeated role in the formation of collagen's helix structure in conjunction with hydroxyproline.[27][31] In the genetic code, glycine is coded by all codons starting with GG, namely GGU, GGC, GGA and GGG.

As a biosynthetic intermediate

In higher eukaryotes, δ-aminolevulinic acid, the key precursor to porphyrins, is biosynthesized from glycine and succinyl-CoA by the enzyme ALA synthase. Glycine provides the central C2N subunit of all purines.[27]

As a neurotransmitter

Glycine is an inhibitory neurotransmitter in the central nervous system, especially in the spinal cord, brainstem, and retina. When glycine receptors are activated, chloride enters the neuron via ionotropic receptors, causing an inhibitory postsynaptic potential (IPSP). Strychnine is a strong antagonist at ionotropic glycine receptors, whereas bicuculline is a weak one. Glycine is a required co-agonist along with glutamate for NMDA receptors. In contrast to the inhibitory role of glycine in the spinal cord, this behaviour is facilitated at the (NMDA) glutamatergic receptors which are excitatory.[32] The LD50 of glycine is 7930 mg/kg in rats (oral),[33] and it usually causes death by hyperexcitability.

Uses

In the US, glycine is typically sold in two grades: United States Pharmacopeia (“USP”), and technical grade. USP grade sales account for approximately 80 to 85 percent of the U.S. market for glycine. If purity greater than the USP standard is needed, for example for intravenous injections, a more expensive pharmaceutical grade glycine can be used. Technical grade glycine, which may or may not meet USP grade standards, is sold at a lower price for use in industrial applications, e.g., as an agent in metal complexing and finishing.[34]

Animal and human foods

Structure of cis-Cu(glycinate)2(H2O).[35]

Glycine is not widely used in foods for its nutritional value, except in infusions. Instead glycine's role in food chemistry is as a flavorant. It is mildly sweet, and it counters the aftertaste of saccharine. It also has preservative properties, perhaps owing to its complexation to metal ions. Metal glycinate complexes, e.g. copper(II) glycinate are used as supplements for animal feeds.[22]

Chemical feedstock

Glycine is an intermediate in the synthesis of a variety of chemical products. It is used in the manufacture of the herbicides glyphosate,[36] iprodione, glyphosine, imiprothrin, and eglinazine.[22] It is used as an intermediate of the medicine such as thiamphenicol.

Laboratory research

Glycine is a significant component of some solutions used in the SDS-PAGE method of protein analysis. It serves as a buffering agent, maintaining pH and preventing sample damage during electrophoresis. Glycine is also used to remove protein-labeling antibodies from Western blot membranes to enable the probing of numerous proteins of interest from SDS-PAGE gel. This allows more data to be drawn from the same specimen, increasing the reliability of the data, reducing the amount of sample processing, and number of samples required. This process is known as stripping.

Presence in space

The presence of glycine outside the earth was confirmed in 2009, based on the analysis of samples that had been taken in 2004 by the NASA spacecraft Stardust from comet Wild 2 and subsequently returned to earth. Glycine had previously been identified in the Murchison meteorite in 1970.[37] The discovery of cometary glycine bolstered the hypothesis of so called soft-panspermia, which claims that the "building blocks" of life are widespread throughout the universe.[38] In 2016, detection of glycine within Comet 67P/Churyumov–Gerasimenko by the Rosetta spacecraft was announced.[39]

The detection of glycine outside the Solar System in the interstellar medium has been debated.[40] In 2008, the Max Planck Institute for Radio Astronomy discovered the spectral lines of a glycine precursor (aminoacetonitrile) in the Large Molecule Heimat, a giant gas cloud near the galactic center in the constellation Sagittarius.[41]

Evolution

Several independent evolutionary studies using different types of data have suggested that glycine belongs to a group of amino acids that constituted the early genetic code.[42][43][44][45] For example, low complexity regions (in proteins), that may resemble the proto-peptides of the early genetic code are highly enriched in glycine.[45]

Presence in foods

Food sources of glycine[46]
Food g/100g
Snacks, pork skins11.04
Sesame seeds flour (low fat)3.43
Beverages, protein powder (soy-based)2.37
Seeds, safflower seed meal, partially defatted2.22
Meat, bison, beef and others (various parts)1.5-2.0
Gelatin desserts1.96
Seeds, pumpkin and squash seed kernels1.82
Turkey, all classes, back, meat and skin1.79
Chicken, broilers or fryers, meat and skin1.74
Pork, ground, 96% lean / 4% fat, cooked, crumbles1.71
Bacon and beef sticks1.64
Peanuts1.63
Crustaceans, spiny lobster1.59
Spices, mustard seed, ground1.59
Salami1.55
Nuts, butternuts, dried1.51
Fish, salmon, pink, canned, drained solids1.42
Almonds1.42
Fish, mackerel0.93
Cereals ready-to-eat, granola, homemade0.81
Leeks, (bulb and lower-leaf portion), freeze-dried0.7
Cheese, parmesan (and others), grated0.56
Soybeans, green, cooked, boiled, drained, without salt0.51
Bread, protein (includes gluten)0.47
Egg, whole, cooked, fried0.47
Beans, white, mature seeds, cooked, boiled, with salt0.38
Lentils, mature seeds, cooked, boiled, with salt0.37

See also

References

  1. ↑ The Merck Index: An Encyclopedia of Chemicals, Drugs, and Biologicals (11th ed.), Merck, 1989, ISBN 091191028X, 4386
  2. ↑ pubchem.ncbi.nlm.nih.gov/compound/750#section=IUPAC-Name&fullscreen=true
  3. ↑ Handbook of Chemistry and Physics, CRC Press, 59th edition, 1978
  4. ↑ "Solubilities and densities". Prowl.rockefeller.edu. Archived from the original on 2017-09-12. Retrieved 2013-11-13.
  5. ↑ Dawson, R.M.C., et al., Data for Biochemical Research, Oxford, Clarendon Press, 1959.
  6. ↑ "Nomenclature and Symbolism for Amino Acids and Peptides". IUPAC-IUB Joint Commission on Biochemical Nomenclature. 1983. Archived from the original on 9 October 2008. Retrieved 5 March 2018.
  7. ↑ "Glycine | Definition of glycine in English by Oxford Dictionaries".
  8. ↑ Plimmer, R.H.A. (1912) [1908]. Plimmer, R.H.A.; Hopkins, F.G. (eds.). The chemical composition of the proteins. Monographs on biochemistry. Vol. Part I. Analysis (2nd ed.). London: Longmans, Green and Co. p. 82. Retrieved January 18, 2010.
  9. ↑ Braconnot, Henri (1820). "Sur la conversion des matières animales en nouvelles substances par le moyen de l'acide sulfurique" [On the conversion of animal materials into new substances by means of sulfuric acid]. Annales de Chimie et de Physique. 2nd series (in French). 13: 113–125. ; see p. 114.
  10. ↑ MacKenzie, Colin (1822). One Thousand Experiments in Chemistry: With Illustrations of Natural Phenomena; and Practical Observations on the Manufacturing and Chemical Processes at Present Pursued in the Successful Cultivation of the Useful Arts …. Sir R. Phillips and Company. p. 557.
  11. ↑ Boussingault (1838). "Sur la composition du sucre de gélatine et de l'acide nitro-saccharique de Braconnot" [On the composition of sugar of gelatine and of nitro-glucaric acid of Braconnot]. Comptes Rendus (in French). 7: 493–495.
  12. ↑ Horsford, E.N. (1847). "Glycocoll (gelatine sugar) and some of its products of decomposition". The American Journal of Science and Arts. 2nd series. 3: 369–381.
  13. ↑ Ihde, Aaron J. (1970). The Development of Modern Chemistry. Courier Corporation. ISBN 9780486642352.
  14. ↑ Berzelius, Jacob (1848). Jahres-Bericht über die Fortschritte der Chemie und Mineralogie (Annual Report on the Progress of Chemistry and Mineralogy). Vol. 47. Tübigen, (Germany): Laupp. p. 654. From p. 654: "Er hat dem Leimzucker als Basis den Namen Glycocoll gegeben. … Glycin genannt werden, und diesen Namen werde ich anwenden." (He [i.e., the American scientist Eben Norton Horsford, then a student of the German chemist Justus von Liebig] gave the name "glycocoll" to Leimzucker [sugar of gelatine], a base. This name is not euphonious and has besides the flaw that it clashes with the names of the rest of the bases. It is compounded from γλυχυς (sweet) and χολλα (animal glue). Since this organic base is the only [one] which tastes sweet, then it can much more briefly be named "glycine", and I will use this name.)
  15. ↑ Nye, Mary Jo (1999). Before Big Science: The Pursuit of Modern Chemistry and Physics, 1800-1940. Harvard University Press. ISBN 9780674063822.
  16. ↑ "glycine". Oxford Dictionaries. Retrieved 2015-12-06.
  17. ↑ Cahours, A. (1858). "Recherches sur les acides amidés" [Investigations into aminated acids]. Comptes Rendus (in French). 46: 1044–1047.
  18. ↑ Okafor, Nduka (2016-03-09). Modern Industrial Microbiology and Biotechnology. CRC Press. ISBN 9781439843239.
  19. ↑ Ingersoll, A. W.; Babcock, S. H. (1932). "Hippuric acid". Organic Syntheses. 12: 40.; Collective Volume, vol. 2, p. 328
  20. ↑ Wiley (2007-12-14). Kirk-Othmer Food and Feed Technology, 2 Volume Set. John Wiley & Sons. ISBN 9780470174487.
  21. ↑ "Glycine Conference (prelim)". USITC. Archived from the original on 2012-02-22. Retrieved 2014-06-13.{{cite web}}: CS1 maint: bot: original URL status unknown (link)
  22. 1 2 3 Drauz, Karlheinz; Grayson, Ian; Kleemann, Axel; Krimmer, Hans-Peter; Leuchtenberger, Wolfgang & Weckbecker, Christoph (2007). "Amino Acids". Ullmann's Encyclopedia of Industrial Chemistry. Weinheim: Wiley-VCH.
  23. ↑ Hart, J. Roger (2005). "Ethylenediaminetetraacetic Acid and Related Chelating Agents". Ullmann's Encyclopedia of Industrial Chemistry. Weinheim: Wiley-VCH. doi:10.1002/14356007.a10_095.
  24. ↑ Ingersoll, A. W.; Babcock, S. H. (1932). "Hippuric Acid". Org. Synth. 12: 40. doi:10.15227/orgsyn.012.0040.
  25. ↑ Herbst, R. M.; Shemin, D. (1939). "Acetylglycine". Org. Synth. 19: 4. doi:10.15227/orgsyn.019.0004.
  26. ↑ Meléndez-Hevia, E; De Paz-Lugo, P; Cornish-Bowden, A; Cárdenas, M. L. (December 2009). "A weak link in metabolism: the metabolic capacity for glycine biosynthesis does not satisfy the need for collagen synthesis". Journal of Biosciences. 34 (6): 853–72. doi:10.1007/s12038-009-0100-9. PMID 20093739. S2CID 2786988.
  27. 1 2 3 4 5 6 7 Nelson, David L.; Cox, Michael M. (2005). Principles of Biochemistry (4th ed.). New York: W. H. Freeman. pp. 127, 675–77, 844, 854. ISBN 0-7167-4339-6.
  28. ↑ Wang, W.; Wu, Z.; Dai, Z.; Yang, Y.; Wang, J.; Wu, G. (2013). "Glycine metabolism in animals and humans: Implications for nutrition and health". Amino Acids. 45 (3): 463–77. doi:10.1007/s00726-013-1493-1. PMID 23615880. S2CID 7577607.
  29. 1 2 Hahn RG (1993). "Dose-dependent half-life of glycine". Urological Research. 21 (4): 289–291. doi:10.1007/BF00307714. PMID 8212419. S2CID 25138444.
  30. ↑ ACS Chem Biol. 2010 Aug 20; 5(8): 787–795. doi: 10.1021/cb100096f
  31. ↑ Szpak, Paul (2011). "Fish bone chemistry and ultrastructure: implications for taphonomy and stable isotope analysis". Journal of Archaeological Science. 38 (12): 3358–3372. doi:10.1016/j.jas.2011.07.022.
  32. ↑ "Recent development in NMDA receptors". Chinese Medical Journal. 2000.
  33. ↑ "Safety (MSDS) data for glycine". The Physical and Theoretical Chemistry Laboratory Oxford University. 2005. Archived from the original on 2007-10-20. Retrieved 2006-11-01.
  34. ↑ "Glycine From Japan and Korea" (PDF). U.S. International Trade Commission. January 2008. Retrieved 2014-06-13.
  35. ↑ Casari, B. M.; Mahmoudkhani, A. H.; Langer, V. (2004). "A Redetermination of cis-Aquabis(glycinato-κ2N,O)copper(II)". Acta Crystallogr. E. 60 (12): m1949–m1951. doi:10.1107/S1600536804030041.
  36. ↑ Stahl, Shannon S.; Alsters, Paul L. (2016-07-13). Liquid Phase Aerobic Oxidation Catalysis: Industrial Applications and Academic Perspectives. John Wiley & Sons. ISBN 9783527690152.
  37. ↑ Kvenvolden, Keith A.; Lawless, James; Pering, Katherine; Peterson, Etta; Flores, Jose; Ponnamperuma, Cyril; Kaplan, Isaac R.; Moore, Carleton (1970). "Evidence for extraterrestrial amino-acids and hydrocarbons in the Murchison meteorite". Nature. 228 (5275): 923–926. Bibcode:1970Natur.228..923K. doi:10.1038/228923a0. PMID 5482102. S2CID 4147981.
  38. ↑ "Building block of life found on comet - Thomson Reuters 2009". Reuters. 18 August 2009. Retrieved 2009-08-18.
  39. ↑ European Space Agency (27 May 2016). "Rosetta's comet contains ingredients for life". Retrieved 2016-06-05.
  40. ↑ Snyder LE, Lovas FJ, Hollis JM, et al. (2005). "A rigorous attempt to verify interstellar glycine". Astrophys J. 619 (2): 914–930. arXiv:astro-ph/0410335. Bibcode:2005ApJ...619..914S. doi:10.1086/426677. S2CID 16286204.
  41. ↑ Staff. "Organic Molecule, Amino Acid-Like, Found In Constellation Sagittarius 27 March 2008 - Science Daily". Retrieved 2008-09-16.
  42. ↑ Trifonov, E.N (December 2000). "Consensus temporal order of amino acids and evolution of the triplet code". Gene. 261 (1): 139–151. doi:10.1016/S0378-1119(00)00476-5. PMID 11164045.
  43. ↑ Higgs, Paul G.; Pudritz, Ralph E. (June 2009). "A Thermodynamic Basis for Prebiotic Amino Acid Synthesis and the Nature of the First Genetic Code". Astrobiology. 9 (5): 483–490. arXiv:0904.0402. Bibcode:2009AsBio...9..483H. doi:10.1089/ast.2008.0280. ISSN 1531-1074. PMID 19566427. S2CID 9039622.
  44. ↑ Chaliotis, Anargyros; Vlastaridis, Panayotis; Mossialos, Dimitris; Ibba, Michael; Becker, Hubert D.; Stathopoulos, Constantinos; Amoutzias, Grigorios D. (2017-02-17). "The complex evolutionary history of aminoacyl-tRNA synthetases". Nucleic Acids Research. 45 (3): 1059–1068. doi:10.1093/nar/gkw1182. ISSN 0305-1048. PMC 5388404. PMID 28180287.
  45. 1 2 Ntountoumi, Chrysa; Vlastaridis, Panayotis; Mossialos, Dimitris; Stathopoulos, Constantinos; Iliopoulos, Ioannis; Promponas, Vasilios; Oliver, Stephen G; Amoutzias, Grigoris D (2019-11-04). "Low complexity regions in the proteins of prokaryotes perform important functional roles and are highly conserved". Nucleic Acids Research. 47 (19): 9998–10009. doi:10.1093/nar/gkz730. ISSN 0305-1048. PMC 6821194. PMID 31504783.
  46. ↑ "National Nutrient Database for Standard Reference". U.S. Department of Agriculture. Archived from the original on 2015-03-03. Retrieved 2009-09-07. {{cite journal}}: Cite journal requires |journal= (help)

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