Estramustine phosphate

Estramustine phosphate
Names
Trade namesEmcyt, Estracyt, others
Other namesEstradiol normustine phosphate; EMP; Leo 299; NSC-89199; Ro 21-8837/001; Estradiol 3-normustine 17β-phosphate; Estradiol 3-(bis(2-chloroethyl)carbamate) 17β-(dihydrogen phosphate)
IUPAC name
  • [(8R,9S,13S,14S,17S)-13-methyl-17-phosphonooxy-6,7,8,9,11,12,14,15,16,17-decahydrocyclopenta[a]phenanthren-3-yl] N,N-bis(2-chloroethyl)carbamate
Clinical data
Drug classChemotherapeutic agent (alkylating agent)[1]
Main usesProstate cancer[2]
Side effectsDiarrhea, heart failure, low red blood cells, breast enlargement, liver problems, blood clots, nausea[3]
WHO AWaReUnlinkedWikibase error: ⧼unlinkedwikibase-error-statements-entity-not-set⧽
Pregnancy
category
  • AU: D
  • US: X (Contraindicated)
    Routes of
    use    		By mouth
    External links
    AHFS/Drugs.comMonograph
    MedlinePlusa608046
    Legal
    License data
    Legal status
    Pharmacokinetics
    Bioavailability44–75% (as estramustine and estromustine)[4]
    Protein binding• Estradiol: 98%[5]
    • Estrone: 96%[5]
    MetabolismLiver, intestines[6][4][7]
    Metabolites• Estramustine[6][4]
    • Estromustine[6][4]
    • Estradiol[6][4]
    • Estrone[6][4]
    • Phosphoric acid[6][4]
    • Normustine[8]
    Elimination half-life• EMP: 1.27 hours[9]
    • Estromustine: 10–14 hrs[4]
    • Estrone: 15–17 hours[4]
    ExcretionBile, feces (2.9–4.8%)[4][7]
    Chemical and physical data
    FormulaC23H32Cl2NO6P
    Molar mass520.38 g·mol−1
    3D model (JSmol)
    SMILES
    • C[C@]12CC[C@H]3[C@H]([C@@H]1CC[C@@H]2OP(=O)(O)O)CCC4=C3C=CC(=C4)OC(=O)N(CCCl)CCCl
    InChI
    • InChI=1S/C23H32Cl2NO6P/c1-23-9-8-18-17-5-3-16(31-22(27)26(12-10-24)13-11-25)14-15(17)2-4-19(18)20(23)6-7-21(23)32-33(28,29)30/h3,5,14,18-21H,2,4,6-13H2,1H3,(H2,28,29,30)/t18-,19-,20+,21+,23+/m1/s1
    • Key:ADFOJJHRTBFFOF-RBRWEJTLSA-N

    Estramustine phosphate (EMP), sold under the brand names Emcyt among others, is a medication used to treat prostate cancer.[2] Specifically it is used when the disease is hormone refractory.[2] It is taken by mouth three to four times per day.[2]

    Common side effects include diarrhea, heart failure, low red blood cells, breast enlargement, liver problems, blood clots, and nausea.[3] Other side effects may include sexual dysfunction, high blood pressure, and angioedema.[2] Use in pregnancy may harm the baby.[2] It is made by joining an estradiol with a nitrogen mustard.[10]

    Estramustine phosphate was introduced for medical use in the early 1970s.[6] It is available in the United States, Canada, the United Kingdom, and other European countries, among others.[1] In the United Kingdom 100 tablets of 140 mg costs the NHS about £170 as of 2021.[3] This amount in the United States costs about 1,600 USD.[11]

    Medical uses

    EMP is indicated, in the United States, for the palliative treatment of metastatic and/or progressive prostate cancer,[7] whereas in the United Kingdom it is indicated for the treatment of unresponsive or relapsing prostate cancer.[12][9][4][13] The medication is usually reserved for use in hormone-refractory cases of prostate cancer, although it has been used as a first-line monotherapy as well.[6] Response rates with EMP in prostate cancer are said to be equivalent to conventional high-dose estrogen therapy.[14]

    Due to its relatively severe side effects and toxicity, EMP has rarely been used in the treatment of prostate cancer.[8] This is especially true in Western countries today.[8] As a result, and also due to the scarce side effects of gonadotropin-releasing hormone modulators (GnRH modulators) like leuprorelin, EMP was almost abandoned.[6] However, encouraging clinical research findings resulted in renewed interest of EMP for the treatment of prostate cancer.[6]

    EMP has been used at doses of 140 to 1,400 mg/day orally.[15] Low doses, such as 280 mg/day, have been found to have comparable effectiveness as higher doses but with improved tolerability and reduced toxicity.[8] Doses of 140 mg/day have been described as a very low dosage.[16] EMP has been used at doses of 240 to 450 mg/day intravenously.[4]

    EMP and other estrogens such as polyestradiol phosphate and ethinylestradiol are far less costly than newer therapies such as GnRH modulators, abiraterone acetate, and enzalutamide.[8][17][18] In addition, estrogens may offer significant benefits over other means of androgen deprivation therapy, for instance in terms of bone loss and fractures, hot flashes, cognition, and metabolic status.[8][18]

    EMP has been used to prevent the testosterone flare at the start of GnRH agonist therapy in men with prostate cancer.[19]

    Estrogen dosages for prostate cancer
    Route/formEstrogenDosage
    OralEstradiol1–2 mg 3x/day
    Conjugated estrogens1.25–2.5 mg 3x/day
    Ethinylestradiol0.15–3 mg/day
    Ethinylestradiol sulfonate1–2 mg 1x/week
    Diethylstilbestrol1–3 mg/day
    Dienestrol5 mg/day
    Hexestrol5 mg/day
    Fosfestrol100–480 mg 1–3x/day
    Chlorotrianisene12–48 mg/day
    Quadrosilan900 mg/day
    Estramustine phosphate140–1400 mg/day
    Transdermal patchEstradiol2–6x 100 μg/day
    Scrotal: 1x 100 μg/day
    IM or SC injectionEstradiol benzoate1.66 mg 3x/week
    Estradiol dipropionate5 mg 1x/week
    Estradiol valerate10–40 mg 1x/1–2 weeks
    Estradiol undecylate100 mg 1x/4 weeks
    Polyestradiol phosphateAlone: 160–320 mg 1x/4 weeks
    With oral EE: 40–80 mg 1x/4 weeks
    Estrone2–4 mg 2–3x/week
    IV injectionFosfestrol300–1200 mg 1–7x/week
    Estramustine phosphate240–450 mg/day
    Note: Dosages are not necessarily equivalent. Sources: See template.

    Dosage

    It is generally started at 560 to 840 mg per day; with a long term dose of 140 to 1,400 mg per day.[3]

    EMP is or has been available in the form of both capsules (140 mg, 280 mg) for oral administration and aqueous solutions (300 mg) for intravenous injection.[15][20][21][22]

    Contraindications

    EMP is contraindicated when used in children, patients hypersensitive to estrogens or nitrogen mustards, those with peptic ulcer (an ulcer in the digestive tract), those with severely compromised liver function, those with weak heart muscle (also known as myocardial insufficiency) and those with thromboembolic disorders or complications related to fluid retention.[12]

    Side effects

    The side effects of EMP overall have been described as relatively severe.[6] The most common side effects of EMP have been reported to be gastrointestinal side effects like nausea, vomiting, and diarrhea, with nausea and vomiting occurring in 40% of men.[23][4] They are usually mild or moderate in severity, and the nausea and vomiting can be managed with prophylactic antiemetic medications.[23] Nonetheless, severe cases of gastrointestinal side effects with EMP may require dose reduction or discontinuation of therapy.[4] Although nausea and vomiting have been reported to be the most common side effects of EMP, gynecomastia (male breast development) has been found to occur in as many as 83% of men treated with EMP, and the incidence of erectile dysfunction is possibly similar to or slightly less than the risk of gynecomastia.[6] As a rule, feminization, a gynoid fat distribution, demasculinization, and impotence are said to occur in virtually or nearly 100% of men treated with high-dose estrogen therapy.[24][25] Decreased sexual activity has also been reported in men treated with EMP.[4] These side effects are due to high estrogen levels and low testosterone levels.[4][6] Prophylactic irradiation of the breasts can be used to decrease the incidence and severity of gynecomastia with estrogens.[24]

    Severe adverse effects of EMP are thromboembolic and cardiovascular complications including pulmonary embolism, deep vein thrombosis, stroke, thrombophlebitis, coronary artery disease (ischemic heart disease; e.g., myocardial infarction), thrombophlebitis, and congestive heart failure with fluid retention.[23][4] EMP produces cardiovascular toxicity similarly to diethylstilbestrol, but to a lesser extent in comparison at low doses (e.g., 280 mg/day oral EMP vs. 1 mg/day oral diethylstilbestrol).[6][26] The prostate cancer disease state also increases the risk of thromboembolism, and combination with docetaxel may exacerbate the risk of thromboembolism as well.[23] Meta-analyses of clinical trials have found that the overall risk of thromboembolism with EMP is 4 to 7%, relative to 0.4% for chemotherapy regimens without EMP.[23] Thromboembolism is the major toxicity-related cause of discontinuation of EMP.[27] Anticoagulant therapy with medications such as aspirin, warfarin, unfractionated and low-molecular-weight heparin, and vitamin K antagonists can be useful for decreasing the risk of thromboembolism with EMP and other estrogens like diethylstilbestrol and ethinylestradiol.[23][28][8]

    Adverse liver function tests are commonly seen with EMP, but severe liver dysfunction is rare with the medication.[4] Central nervous system side effects are rarely seen with EMP, although enlarged ventricles and neuronal pigmentation have been reported in monkeys treated with very high doses of EMP (20–140 mg/kg/day) for 3 to 6 months.[4] EMP does not appear to have cytostatic effects in normal brain tissue.[4] In women treated with EMP in clinical studies, a few instances of minor gynecological hemorrhages have been observed.[4] EMP is described as relatively well tolerated among cytostatic antineoplastic and nitrogen-mustard agents, rarely or not at all being associated with significant hematologic toxicity such as myelosuppression (bone marrow suppression), gastrointestinal toxicity, or other more marked toxicity associated with such agents.[9][4][29] In contrast to most other cytostatic agents, which often cause myelosuppression, leukopenia (decreased white blood cell count), and neutropenia (decreased neutrophil count), EMP actually produces leukocytosis (increased white blood cell count) as a side effect.[30][31]

    In a small low-dose study using 280 mg/day oral EMP for 150 days, tolerability was significantly improved, with gastrointestinal irritation occurring in only 15% of men, and there was no incidence of severe cardiovascular toxicity or deep vein thrombosis.[6][8] In addition, no other side effects besides slight transient elevated liver enzymes were observed.[6] These findings suggest that lower doses of oral EMP may be a safer option than higher doses for the treatment of prostate cancer.[8]

    Side effects of estramustine phosphate (EMC)
    System organ class Very common (≥10%) Common (1–10%) Frequency unknownc
    Blood and lymphatic system disorders Anemia; Leukopenia Thrombocytopenia
    Immune system disorders Hypersensitivity
    Metabolism and nutrition disorders Fluid retention
    Psychiatric disorders Confusional state; Depression
    Nervous system disorders Lethargy; Headache
    Cardiac disorders Congestive heart failure Myocardial infarction Myocardial ischemia
    Vascular disorders Embolism Hypertension
    Gastrointestinal disorders Nauseab; Vomitingb; Diarrheab
    Hepatobiliary disorders Abnormal hepatic function
    Skin and subcutaneous tissue disorders Angioedemac; Allergic dermatitis
    Musculoskeletal and connective tissue disorders Muscle weakness
    Reproductive system and breast disorders Gynecomastia Erectile dysfunction
    General disorders and administration site conditions Injection site thrombosis (IV solution)
    Footnotes: a = Could not be estimated from available data. b = Especially during the first 2 weeks of therapy. c = Angioedema (Quincke edema, larynx edema) can occur. Often occurs in combination with ACE inhibitors. EMP should be immediately discontinued if angioedema occurs. Sources: See template.
    Side effects of estramustine phosphate (FDA)
    Side effectEstramustine phosphate (oral
    11.5–15.9 mg/kg/day) (n = 93) (%)    		Diethylstilbestrol (oral
    3.0 mg/day) (n = 93) (%)
    Cardiovascular and respiratory
    Cardiac arrest02
    Cerebrovascular accident (stroke)20
    Myocardial infarction (heart attack)31
    Thrombophlebitis37
    Pulmonary embolism25
    Congestive heart failure32
    Edema (swelling)1917
    Dyspnea (shortness of breath)113
    Leg cramps811
    Upper respiratory discharge11
    Hoarseness10
    Gastrointestinal
    Nausea158
    Diarrhea1211
    Minor gastrointestinal upset116
    Anorexia (appetite loss)43
    Flatulence20
    Vomiting11
    Gastrointestinal bleeding10
    Burning throat10
    Thirst10
    Integumentary
    Rash14
    Pruritus (itching)22
    Dry skin20
    Pigment changes03
    Easy bruising30
    Flushing10
    Night sweats01
    Peeling of fingertip skin10
    Thinning hair11
    Breast changes
    Breast tenderness6664
    Breast enlargement (gynecomastia or male breast development)
      Mild6054
      Moderate1016
      Marked05
    Miscellaneous
    Lethargy alone43
    Depression02
    Emotional lability20
    Insomnia30
    Headache11
    Anxiety10
    Chest pain11
    Hot flashes01
    Eye pain01
    Lacrimation (eye tears)11
    Tinnitus01
    Laboratory abnormalities
    Hematologic (blood)
    Leukopenia (low white blood cells)42
    Thrombopenia (low platelets)12
    Hepatic (liver)
    Bilirubin alone15
    Bilirubin and LDH01
    Bilirubin and AST21
    Bilirubin, LDH, and SGOT20
    LDH and/or SGOT3128
    Miscellaneous
    Transient hypercalcemia (high calcium)01
    Sources: [22]

    Overdose

    There has been no clinical experience with overdose of EMP.[22] Overdose of EMP may result in pronounced manifestations of the known adverse effects of the medication.[22] There is no specific antidote for overdose of EMP.[12] In the event of overdose, gastric lavage should be used to evacuate gastric contents as necessary and treatment should be symptom-based and supportive.[22][12] In the case of dangerously low counts of red blood cells, white blood cells, or platelets, whole blood may be given as needed.[12] Liver function should be monitored with EMP overdose.[12] After an overdose of EMP, hematological and hepatic parameters should continue to be monitored for at least 6 weeks.[22]

    EMP has been used at high doses of as much as 1,260 mg/day by the oral route and 240 to 450 mg/day by intravenous injection.[6][4]

    Interactions

    EMP has been reported to increase the efficacy and toxicity of tricyclic antidepressants like amitriptyline and imipramine.[12] When products containing calcium, aluminium, and/or magnesium, such as dairy products like milk, various foods dietary supplements, and antacids, are consumed concomitantly with EMP, an insoluble chelate complex/phosphate salt between EMP and these metals can be formed, and this can markedly impair the absorption and hence oral bioavailability of EMP.[6][4][12] There may be an increased risk of angioedema in those concurrently taking ACE inhibitors.[12]

    Pharmacology

    Pharmacodynamics

    See also: Pharmacodynamics of estradiol
    Estramustine, the major active cytostatic form of estramustine phosphate.
    Estradiol, the major active estrogenic form of estramustine phosphate.

    EMP, also known as estradiol normustine phosphate, is a combined estrogen ester and nitrogen mustard ester.[4][6][32] It consists of estradiol, an estrogen, linked with a phosphate ester as well as an ester of normustine, a nitrogen mustard.[4][6][32] In terms of its pharmacodynamic effects, EMP is a prodrug of estramustine, estromustine, and estradiol.[4][6] As a prodrug of estradiol, EMP is an estrogen and hence an agonist of the estrogen receptors.[4][5] EMP itself has only very weak affinity for the estrogen receptors.[4] The medication is of about 91% higher molecular weight than estradiol due to the presence of its C3 normustine and C17β phosphate esters.[33][1] Because EMP is a prodrug of estradiol, it may be considered to be a natural and bioidentical form of estrogen,[32] although it does have additional cytostatic activity via estramustine and estromustine.[4][6]

    EMP acts by a dual mechanism of action: 1) direct cytostatic activity via a number of actions; and 2) as a form of high-dose estrogen therapy via estrogen receptor-mediated antigonadotropic and functional antiandrogenic effects.[4][6][32] The antigonadotropic and functional antiandrogenic effects of EMP consist of strong suppression of gonadal androgen production and hence circulating levels of androgens such as testosterone; greatly increased levels of sex hormone-binding globulin and hence a decreased fraction of free androgens in the circulation; and direct antiandrogenic actions in prostate cells.[29][4][6][8][34][35][36] The free androgen index with oral EMP has been found to be on average 4.6-fold lower than with orchiectomy.[35] As such, EMP therapy results in considerably stronger androgen deprivation than orchiectomy.[36] Metabolites of EMP, including estramustine, estromustine, estradiol, and estrone, have been found to act as weak antagonists of the androgen receptor (EC50 = 0.5–3.1 μM), although the clinical significance of this is unknown.[37][34][6][4]

    Extremely high levels of estradiol and estrone occur during EMP therapy.[6][8] The estrogenic metabolites of EMP are responsible for its most common adverse effects and its cardiovascular toxicity.[4] EMP has been described as having relatively weak estrogenic effects in some publications.[9][29] However, it has shown essentially the same rates and degrees of estrogenic effects, such as breast tenderness, gynecomastia, cardiovascular toxicity, changes in liver protein synthesis, and testosterone suppression, as high-dose diethylstilbestrol and ethinylestradiol in clinical studies.[22][8][29][36][38] The notion that EMP has relatively weak estrogen activity may have been based on animal research, which found that EMP had 100-fold lower uterotrophic effects than estradiol in rats, and may also not have taken into account the very high doses of EMP used clinically in humans.[38][39]

    The mechanism of action of the cytostatic effects of EMP is complex and only partially understood.[4] EMP is considered to mainly be a mitotic inhibitor, inhibiting mechanisms involved in the mitosis phase of the cell cycle.[4][8] Specifically, it binds to microtubule-associated proteins and/or to tubulin and produces depolymerization of microtubules (Kd = 10–20 μM for estramustine), resulting in the arrest of cell division in the G2/M phase (specifically metaphase).[4][8][40] EMP was originally thought to mediate its cytostatic effects as a prodrug of normustine, a nitrogen mustard, and hence was thought to be an alkylating antineoplastic agent.[6][13][9][32] However, subsequent research has found that EMP is devoid of alkylating actions, and that the influence of EMP on microtubules is mediated by intact estramustine and estromustine, with normustine or estradiol alone having only minor or negligible effects.[4][6][41] As such, the unique properties of the estramustine and estromustine structures, containing a carbamate-ester bond, appear to be responsible for the cytostatic effects of EMP.[4] In addition to its antimitotic actions, EMP has also been found to produce other cytostatic effects, including induction of apoptosis, interference with DNA synthesis, nuclear matrix interaction, cell membrane alterations, induction of reactive oxygen species (free oxygen radicals), and possibly additional mechanisms.[4][8] EMP has been found to have a radiosensitizing effect in prostate cancer and glioma cells, improving sensitivity to radiation therapy as well.[4]

    The cytostatic metabolites of EMP are accumulated in tissues in a selective manner, for instance in prostate cancer cells.[9][4][8] This may be due to the presence of a specific estramustine-binding protein (EMBP) (Kd = 10–35 nM for estramustine), also known as prostatin or prostatic secretion protein (PSP), which has been detected in prostate cancer, glioma, melanoma, and breast cancer cells.[4][8][42] Because of its tissue selectivity, EMP is said to produce minimal cytostatic effects in healthy tissues, and its tissue selectivity may be responsible for its therapeutic cytostatic efficacy against prostate cancer cells.[9][8][4]

    EMP was originally developed as a dual ester prodrug of an estrogen and normustine as a nitrogen mustard alkylating antineoplastic agent which, due to the affinity of the estrogen moiety for estrogen receptors, would be selectively accumulated in estrogen target tissues and hence estrogen receptor-positive tumor cells.[8][32][5] Consequentially, it was thought that EMP would preferentially deliver the alkylating normustine moiety to these tissues, allowing for reduced cytostatic effects in healthy tissues and hence improved efficacy and tolerability.[8] However, subsequent research found that there is very limited and slow cleavage of the normustine ester and that EMP is devoid of alkylating activity.[8][4][6][29] In addition, it appears that estramustine and estromustine may be preferentially accumulated in estrogen target tissues not due to affinity for the estrogen receptors, but instead due to affinity for the distinct EMBP.[4][6]

    Extremely high, pregnancy-like levels of estradiol may be responsible for the leukocytosis (increased white blood cell count) that is observed in individuals treated with EMP.[30][31] This side effect is in contrast to most other cytotoxic agents, which instead cause myelosuppression (bone marrow suppression), leukopenia (decreased white blood cell count), and neutropenia (decreased neutrophil count).

    Antigonadotropic effects

    EMP at a dosage 280 mg/day has been found to suppress testosterone levels in men into the castrate range (to 30 ng/dL) within 20 days and to the low castrate range (to 10 ng/dL) within 30 days.[6] Similarly, a dosage of 70 mg/day EMP suppressed testosterone levels into the castrate range within 4 weeks.[6]

    Pharmacokinetics

    See also: Pharmacokinetics of estradiol
    Estradiol and testosterone levels during therapy with 280 mg/day oral EMP in men with prostate cancer (n = 11).[6]
    Pharmacokinetics of estromustine after a single dose of EMP in men with prostate cancer (n = 5)
    ParameterIV 300 mgOral 420 mg
    Cmax506 ± 61 ng/mL362 ± 38 ng/mL
    Tmax2.6 ± 0.4 hours2.2 ± 0.2 hours
    t1/210.3 ± 0.95 hours13.6 ± 3.09 hours
    AUC0–324.82 ± 0.622.88 ± 0.34
    Bioavailability100.0%43.7% ± 4.6%
    Sources: [29]

    Levels of EMP metabolites during continuous therapy with 560 mg/day oral EMP in men
    MetabolitePlasmaRatio
    Estramustine20,000–23,000 pg/mL1:9.6–9.8
    Estromustine191,000–267,000 pg/mL
    Estradiol4,900–9,000 pg/mL1:9.4–11.8
    Estrone71,000–85,000 pg/mL
    Sources: [29]

    Upon oral ingestion, EMP is rapidly and completely dephosphorylated by phosphatases into estramustine during the first pass in the gastrointestinal tract.[4][8][9][43] Estramustine is also partially but considerably oxidized into estromustine by 17β-hydroxysteroid dehydrogenases during the first pass.[9][4][44][45] As such, EMP reaches the circulation as estramustine and estromustine, and the major metabolite of EMP is estromustine.[4][44] A limited quantity of approximately 10 to 15% of estramustine and estromustine is further slowly metabolized via hydrolysis of the normustine ester into estradiol and estrone, respectively.[4][8][29] This reaction is believed to be catalyzed by carbamidases, although the genes encoding the responsible enzymes have not been characterized.[4][46][47] The circulating levels of normustine formed from EMP are insignificant.[41][48] Release of nitrogen mustard gas from normustine via cleavage of the carboxylic acid group has not been demonstrated and does not seem to occur.[40][29]

    The oral bioavailability of EMP is low, which is due to profound first-pass metabolism; specifically, dephosphorylation of EMP.[4] The oral bioavailability of EMP specifically as estramustine and estromustine is 44 to 75%, suggesting that absorption may be incomplete.[4] In any case, there is a linear relationship between the oral dose of EMP and circulating levels of estramustine and estromustine.[4] Consumption of calcium, aluminium, or magnesium with oral EMP can markedly impair its bioavailability due to diminished absorption from the intestines, and this may interfere with its therapeutic effectiveness at low doses.[6][12]

    Following a single oral dose of 420 mg EMP in men with prostate cancer, maximal levels of estromustine were 310 to 475 ng/mL (475,000 pg/mL) and occurred after 2 to 3 hours.[4] Estradiol levels with 280 mg/day oral EMP have been found to increase to very high concentrations within one week of therapy.[6] In one study, levels of estradiol were over 20,000 pg/mL after 10 days, were about 30,000 pg/mL after 30 days, and peaked at about 40,000 pg/mL at 50 days.[6] Another study found lower estradiol levels of 4,900 to 9,000 pg/mL during chronic therapy with 560 mg/day oral EMP.[29] An additional study found estradiol levels of about 17,000 pg/mL with 140 mg/day oral EMP and 38,000 pg/mL with 280 mg/day oral EMP. The circulating levels of estradiol and estrone during EMP therapy have been reported to exceed normal levels in men by more than 100- and 1,000-fold, respectively.[8][29] Levels of estramustine and estradiol in the circulation are markedly lower than those of estromustine and estrone, respectively, with a ratio of about 1:10 in both cases.[4][29] Nonetheless, estradiol levels during EMP therapy appear to be similar to those that occur in mid-to-late pregnancy, which range from 5,000 to 40,000 pg/mL.[49] No unchanged EMP is seen in the circulation with oral administration.[4]

    The pharmacokinetics of EMP are different with intravenous injection.[4] Following a single intravenous injection of 300 mg EMP, levels of EMP were higher than those of its metabolites for the first 8 hours.[4] This is likely due to the bypassing of first-pass metabolism.[4] However, by 24 hours after the dose, unchanged EMP could no longer be detected in the circulation.[4] The clearance of EMP from blood plasma is 4.85 ± 0.684 L/h.[4] The volumes of distribution of EMP with intravenous injection were small; under a two-compartment model, the volume of distribution for the central compartment was 0.043 L/kg and for the peripheral compartment was 0.11 L/kg.[4] The plasma protein binding of EMP is high.[4] Estramustine is accumulated in tumor tissue, for instance prostate cancer and glioma tissue, with estramustine levels much higher in these tissues than in plasma (e.g., 6.3- and 15.9-fold, respectively).[4] Conversely, levels of estromustine in tumor versus plasma are similar (1.0- and 0.5-fold, respectively).[4] Estramustine and estromustine appear to accumulate in adipose tissue.[4]

    The elimination half-life of estromustine with oral EMP was 13.6 hours on average, with a range of 8.8 to 22.7 hours.[4] Conversely, the elimination half-life of estromustine with intravenous injection was 10.3 hours, with a range of 7.36 to 12.3 hours.[4] For comparison, the corresponding elimination half-lives of estrone were 16.5 and 14.7 hours for oral and intravenous administration, respectively.[4] Estramustine and estromustine are mainly excreted in bile and hence in feces.[4][29] They are not believed to be excreted in urine.[4]

    Metabolism of oral estramustine phosphate in humans.[9][4][29][46][47]

    Chemistry

    See also: Estrogen ester, List of estrogen esters § Estradiol esters, and List of hormonal cytostatic antineoplastic agents

    EMP, also known as estradiol 3-normustine 17β-phosphate or as estradiol 3-(bis(2-chloroethyl)carbamate) 17β-(dihydrogen phosphate), is a synthetic estrane steroid and a derivative of estradiol.[33][1] It is an estrogen ester; specifically, EMP is a diester of estradiol with a C3 normustine (nitrogen mustard–carbamate moiety) ester and a C17β phosphate ester.[33][1] EMP is provided as the sodium or meglumine salt.[33][1][20] EMP is similar as a compound to other estradiol esters such as estradiol sulfate and estradiol valerate, but differs in the presence of its nitrogen mustard ester moiety.[33][1] Antineoplastic agents related to EMP, although none of them were marketed, include alestramustine, atrimustine, cytestrol acetate, estradiol mustard, ICI-85966, and phenestrol.[33][1]

    Due to its hydrophilic phosphate ester moiety, EMP is a readily water-soluble compound.[50][51][52] This is in contrast to most other estradiol esters, which are fatty acid esters and lipophilic compounds that are not particularly soluble in water.[5] Unlike EMP, estramustine is highly lipophilic, practically insoluble in water, and non-ionizable.[15] The phosphate ester of EMP was incorporated into the molecule in order to increase its water solubility and allow for intravenous administration.[22]

    Estramustine, as a non phosphate, was never marketed.[53][54]

    Structural properties of selected estradiol esters
    EstrogenStructureEster(s)Relative
    mol. weight    		Relative
    E2 contentb    		log Pc
    Position(s)Moiet(ies)TypeLengtha
    Estradiol
    		––––1.001.004.0
    Estradiol acetate
    		C3Ethanoic acidStraight-chain fatty acid21.150.874.2
    Estradiol benzoate
    		C3Benzoic acidAromatic fatty acid– (~4–5)1.380.724.7
    Estradiol dipropionate
    		C3, C17βPropanoic acid (×2)Straight-chain fatty acid3 (×2)1.410.714.9
    Estradiol valerate
    		C17βPentanoic acidStraight-chain fatty acid51.310.765.6–6.3
    Estradiol benzoate butyrate
    		C3, C17βBenzoic acid, butyric acidMixed fatty acid– (~6, 2)1.640.616.3
    Estradiol cypionate
    		C17βCyclopentylpropanoic acidCyclic fatty acid– (~6)1.460.696.9
    Estradiol enanthate
    		C17βHeptanoic acidStraight-chain fatty acid71.410.716.7–7.3
    Estradiol dienanthate
    		C3, C17βHeptanoic acid (×2)Straight-chain fatty acid7 (×2)1.820.558.1–10.4
    Estradiol undecylate
    		C17βUndecanoic acidStraight-chain fatty acid111.620.629.2–9.8
    Estradiol stearate
    		C17βOctadecanoic acidStraight-chain fatty acid181.980.5112.2–12.4
    Estradiol distearate
    		C3, C17βOctadecanoic acid (×2)Straight-chain fatty acid18 (×2)2.960.3420.2
    Estradiol sulfate
    		C3Sulfuric acidWater-soluble conjugate–1.290.770.3–3.8
    Estradiol glucuronide
    		C17βGlucuronic acidWater-soluble conjugate–1.650.612.1–2.7
    Estramustine phosphated
    		C3, C17βNormustine, phosphoric acidWater-soluble conjugate–1.910.522.9–5.0
    Polyestradiol phosphatee
    		C3–C17βPhosphoric acidWater-soluble conjugate–1.23f0.81f2.9g
    Footnotes: a = Length of ester in carbon atoms for straight-chain fatty acids or approximate length of ester in carbon atoms for aromatic or cyclic fatty acids. b = Relative estradiol content by weight (i.e., relative estrogenic exposure). c = Experimental or predicted octanol/water partition coefficient (i.e., lipophilicity/hydrophobicity). Retrieved from PubChem, ChemSpider, and DrugBank. d = Also known as estradiol normustine phosphate. e = Polymer of estradiol phosphate (~13 repeat units). f = Relative molecular weight or estradiol content per repeat unit. g = log P of repeat unit (i.e., estradiol phosphate). Sources: See individual articles.

    History

    EMP was first synthesized in the mid-1960s and was patented in 1967.[40] It was initially developed for the treatment of breast cancer.[4] The idea for EMP was inspired by the uptake and accumulation of radiolabeled estrogens into breast cancer tissue.[4] However, initial clinical findings of EMP in women with breast cancer were disappointing.[4] Subsequently, radiolabeled EMP was found to be taken up into and accumulated rat prostate gland, and this finding culminated in the medication being repurposed for the treatment of prostate cancer.[4][6] EMP was introduced for medical use in the treatment of this condition in the early 1970s, and was approved in the United States for this indication in 1981.[4][6][55] EMP was originally introduced for use by intravenous injection.[29] Subsequently, an oral formulation was introduced, and the intravenous preparation was almost abandoned in favor of the oral version.[29]

    Society and culture

    Generic names

    EMP is provided as the sodium salt for oral administration, which has the generic names estramustine phosphate sodium (USAN) and estramustine sodium phosphate (BANM, JAN), and as the meglumine salt for intravenous administration, which has the generic name estramustine phosphate meglumine.[20][33][1][56][57] The INNM is estramustine phosphate.[33] The name estramustine phosphate is a contraction of estradiol normustine phosphate.[33][57] EMP is also known by its former developmental code names Leo 299, Ro 21-8837, and Ro 21-8837/001.[33][1][57]

    Brand names

    EMP is most commonly marketed under the brand names Estracyt and Emcyt, but has also been sold under a number of other brand names, including Amsupros, Biasetyl, Cellmustin, Estramustin HEXAL, Estramustina Filaxis, Estranovag, Multosin, Multosin Injekt, Proesta, Prostamustin, and Suloprost.[1][57][20]

    Availability

    See also: List of estrogens available in the United States

    EMP is marketed in the United States,[58] Canada, and Mexico under the brand name Emcyt, whereas the medication is marketed under the brand name Estracyt in the United Kingdom and elsewhere throughout Europe as well as in Argentina, Chile, and Hong Kong.[1] It has been discontinued in a number of countries, including Australia, Brazil, Ireland, and Norway.[59]

    Research

    EMP has been studied in the treatment of other cancers such as glioma and breast cancer.[4] It has been found to slightly improve quality of life in people with glioma during the first 3 months of therapy.[4]

    References

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    2. 1 2 3 4 5 6 "Estramustine Monograph for Professionals". Drugs.com. Archived from the original on 7 January 2022. Retrieved 16 December 2021.
    3. 1 2 3 4 BNF 81: March-September 2021. BMJ Group and the Pharmaceutical Press. 2021. p. 940. ISBN 978-0857114105.
    4. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 Bergenheim AT, Henriksson R (February 1998). "Pharmacokinetics and pharmacodynamics of estramustine phosphate". Clin Pharmacokinet. 34 (2): 163–72. doi:10.2165/00003088-199834020-00004. PMID 9515186. S2CID 1943973.
    5. 1 2 3 4 5 Kuhl H (2005). "Pharmacology of estrogens and progestogens: influence of different routes of administration" (PDF). Climacteric. 8 Suppl 1: 3–63. doi:10.1080/13697130500148875. PMID 16112947. S2CID 24616324. Archived (PDF) from the original on 2016-08-22. Retrieved 2021-08-24.
    6. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 Kitamura T (February 2001). "Necessity of re-evaluation of estramustine phosphate sodium (EMP) as a treatment option for first-line monotherapy in advanced prostate cancer". Int. J. Urol. 8 (2): 33–6. doi:10.1046/j.1442-2042.2001.00254.x. PMID 11240822. S2CID 43384150.
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    8. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Inoue, Takahiro (2018). "Role of Estramustine Phosphate and Other Estrogens for Castration-Resistant Prostate Cancer". Hormone Therapy and Castration Resistance of Prostate Cancer. pp. 249–256. doi:10.1007/978-981-10-7013-6_26. ISBN 978-981-10-7012-9.
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    10. ↑ "Drug Information Portal - U.S. National Library of Medicine - Quick Access to Quality Drug Information". druginfo.nlm.nih.gov. Archived from the original on 11 January 2022. Retrieved 16 December 2021.
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    12. 1 2 3 4 5 6 7 8 9 10 "Estracyt Capsules - Summary of Product Characteristics (SPC)". electronic Medicines Compendium. Pfizer Limited. 12 August 2013. Archived from the original on 22 February 2014. Retrieved 8 February 2014.
    13. 1 2 Simpson, D; Wagstaff, AJ (2003). "Estramustine Phosphate Sodium". American Journal of Cancer. 2 (5): 373–390. doi:10.2165/00024669-200302050-00013. S2CID 70507026.
    14. ↑ Aurel Lupulescu (24 October 1990). Hormones and Vitamins in Cancer Treatment. CRC Press. pp. 40–. ISBN 978-0-8493-5973-6. Archived from the original on 7 January 2022. Retrieved 24 August 2021.
    15. 1 2 3 Valentino Stella; Ronald Borchardt; Michael Hageman; Reza Oliyai, Hans Maag, Jefferson Tilley (12 March 2007). Prodrugs: Challenges and Rewards. Springer Science & Business Media. pp. 174–. ISBN 978-0-387-49782-2. Archived from the original on 7 January 2022. Retrieved 24 August 2021.{{cite book}}: CS1 maint: multiple names: authors list (link)
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    17. ↑ Sayed Y, Taxel P (2003). "The use of estrogen therapy in men". Curr Opin Pharmacol. 3 (6): 650–4. doi:10.1016/j.coph.2003.07.004. PMID 14644018.
    18. 1 2 Hedlund PO, Henriksson P (2000). "Parenteral estrogen versus total androgen ablation in the treatment of advanced prostate carcinoma: effects on overall survival and cardiovascular mortality. The Scandinavian Prostatic Cancer Group (SPCG)-5 Trial Study". Urology. 55 (3): 328–33. doi:10.1016/s0090-4295(99)00580-4. PMID 10699602.
    19. ↑ Shimizu TS, Shibata Y, Jinbo H, Satoh J, Yamanaka H (1995). "Estramustine phosphate for preventing flare-up in luteinizing hormone-releasing hormone analogue depot therapy". Eur. Urol. 27 (3): 192–5. doi:10.1159/000475159. PMID 7541359.
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    27. ↑ Fizazi K, Le Maitre A, Hudes G, Berry WR, Kelly WK, Eymard JC, Logothetis CJ, Pignon JP, Michiels S (2007). "Addition of estramustine to chemotherapy and survival of patients with castration-refractory prostate cancer: a meta-analysis of individual patient data". Lancet Oncol. 8 (11): 994–1000. doi:10.1016/S1470-2045(07)70284-X. PMID 17942366.
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    29. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 Gunnarsson PO, Forshell GP (June 1984). "Clinical pharmacokinetics of estramustine phosphate". Urology. 23 (6 Suppl): 22–7. doi:10.1016/S0090-4295(84)80093-X. PMID 6375076.
    30. 1 2 Alexander NC, Hancock AK, Masood MB, Peet BG, Price JJ, Turner RL, Stone J, Ward AJ (March 1979). "Estracyt in advanced carcinoma of the breast: a phase II study". Clin Radiol. 30 (2): 139–47. doi:10.1016/S0009-9260(79)80133-6. PMID 86404.
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    32. 1 2 3 4 5 6 Michael Oettel; Ekkehard Schillinger (6 December 2012). Estrogens and Antiestrogens II: Pharmacology and Clinical Application of Estrogens and Antiestrogen. Springer Science & Business Media. pp. 540–. ISBN 978-3-642-60107-1.
    33. 1 2 3 4 5 6 7 8 9 10 J. Elks (14 November 2014). The Dictionary of Drugs: Chemical Data: Chemical Data, Structures and Bibliographies. Springer. pp. 502–503. ISBN 978-1-4757-2085-3.
    34. 1 2 Wang LG, Liu XM, Kreis W, Budman DR (May 1998). "Androgen antagonistic effect of estramustine phosphate (EMP) metabolites on wild-type and mutated androgen receptor". Biochem. Pharmacol. 55 (9): 1427–33. doi:10.1016/S0006-2952(97)00657-6. PMID 10076535.
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    37. ↑ Olson KB, Pienta KJ (May 2000). "Recent advances in chemotherapy for advanced prostate cancer". Curr Urol Rep. 1 (1): 48–56. doi:10.1007/s11934-000-0035-z. PMID 12084341. S2CID 31084737.
    38. 1 2 Daehlin L, Damber JE, von Schoultz B, Bergman B (August 1986). "The oestrogenic effects of ethinyl oestradiol/polyoestradiol phosphate and estramustine phosphate in patients with prostatic carcinoma. A comparative study of oestrogen sensitive liver proteins, gonadotrophins and prolactin". Br J Urol. 58 (4): 412–6. doi:10.1111/j.1464-410X.1986.tb09095.x. PMID 3092893.
    39. ↑ Fredholm, B., Jensen, G., Lindskog, M., & Muntzing, J. (1974, January). Effects of estramustine phosphate (Estracyt) on growth of DMBA-induced mammary tumors in rats. In Acta Pharmacologica et Toxicologica (Vol. 35, pp. 28-28). 35 Norre Sogade, PO Box 2148, DK-1016 Copenhagen, Denmark: Munksgaard Int Publ Ltd.
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